天津科技大学学报2016，Vol.31Issue(4)：9-14,6.DOI:10.13364/j.issn.1672-6510.20150182
利用谷氨酸棒杆菌转化法合成4-羟基异亮氨酸
Synthesis of 4-hydroxyisoleucine with Corynebacterium glutamicum via Biotransformation
摘要
Abstract
4-hydroxyisoleucine(4-HIL)possesses glucose-dependent insulinotropic activities .L-isoleucine dioxygenase encoded gene ido from Bacillus thuringiensis TCCC11826 was cloned and expressed in L-isoleucine produced strain Cory-nebacterium glutamicum YILW to synthesize 4-hydroxyisoleucine via biotransformation.The effect ofα-ketoglutarate(α-KG)and Fe2+on 4-HIL synthesis was also studied.C.glutamicum YILW-IDO expressed active IDO was constructed,which could directly convert its endogenous L-isoleucine andα-ketoglutarate in the medium into 4-HIL.Moreover,4-HIL synthesis could be affected by α-KG and Fe2+.After 50,h fermentation ,(35.7 ± 1.0)mmol/L 4-HIL was produced by adding 40,mmol/Lα-KG and 4,mmol/L Fe2+.This research can lay a theoretical foundation for the microbial manufacture technol-ogy of 4-HIL and amino acid derivatives.关键词
4-羟基异亮氨酸/L-异亮氨酸羟化酶/谷氨酸棒状杆菌/微生物转化Key words
4-hydroxyisoleucine/L-isoleucine dioxygenase/Corynebacterium glutamicum/biotransformation分类
生物科学引用本文复制引用
温冰,张成林,麻杰,陈鹏杰,谢希贤,徐庆阳,陈宁..利用谷氨酸棒杆菌转化法合成4-羟基异亮氨酸[J].天津科技大学学报,2016,31(4):9-14,6.基金项目
国家自然科学基金资助项目(31300069)；天津市大学生创新创业训练计划资助项目(201510057063) （31300069）
