天津科技大学学报2016，Vol.31Issue(4)：25-29,5.DOI:10.13364/j.issn.1672-6510.20150117
灰树花胞外多糖的分离纯化及免疫调节作用
The Isolation,Purification and Immunomodulatory Activity of Extracellular Polysaccharide from Grifola frondosa
摘要
Abstract
An extracellular polysaccharide(EXGFP)was obtained by submerged culture of Grifola frondosa,and its prop-erty and immunomodulatory activity were investigated.The final purified fraction,EXGFP-A,was obtained by using alcohol for precipitation,enzymes-sevag for deproteinization and Sephadex G-100,gel column for further isolation and purifica-tion.The purity of EXGFP-A was tested with Sepharose 4B gel column and HPLC.The results show that EXGFP-A is a homogeneous polysaccharide,and its purity is about 92.68%,.Tests of its physicochemical properties indicate that EXGFP-A is a non-starch neutral polysaccharide,excludes uronic acid and polyphenols,and containsα-D-glucoside bond and pyranose ring.The result of MTT assay shows that the cell proliferation index reached a maximum of 137.5%, when EXGFP-A was at a concentration of 80,µg/mL and the treatment time was 48,h.The neutral red phagocytosis experiment indicates that the phagocytic activity of RAW264.7,cells was significantly enhanced by EXGFP-A .According to scanning electron microscope,the activation of RAW264.7,cells could also be promoted by adding EXGFP-A.关键词
灰树花/胞外多糖/分离纯化/小鼠巨噬细胞RAW264.7/免疫活性Key words
Grifola frondosa/extracellular polysaccharide/purification/mouse macrophage RAW264.7/immunomodula-tory activity分类
医药卫生引用本文复制引用
韩丽荣,程代,孟梦,王莉蕊,王春玲..灰树花胞外多糖的分离纯化及免疫调节作用[J].天津科技大学学报,2016,31(4):25-29,5.基金项目
国家科技支撑计划资助项目(2012BAD33B04)；天津市应用基础与前沿技术研究计划资助项目(13JCZDJC29800) （2012BAD33B04）
