现代妇产科进展2016，Vol.25Issue(8)：561-565,5.DOI:10.13283/j.cnki.xdfckjz.2016.08.001
COX2促进卵巢癌细胞迁移及相关机制的研究
The effects and related mechanisms cyclooxygenase-2 act in the migration of ovarian cancer cells
摘要
Abstract
Objective:To examine whether COX2 could accelerate proliferation and metastases of ovarian cancer cells and its related mechanisms. Methods:We created Lenti-COX2-EGFP and then transfected ovarian cancer cells. After that, we evaluated the effect of COX2 on proliferation and metastases of ovarian cancer via PCR,Western blot,qRT-PCR,CCK-8 test and healing test. Results:Compare with control group,after up-regulating COX2 expres-sion,cell proliferation and migration accelerated. The expressions of Snail and Slug up-regula-ted,resulting in E-cadherin expression down-regulating and Vimentin expression up-regulating. Celecoxib can inhibit these affections. All of the differences were significant. Conclusion:COX2 can accelerate cell proliferation and EMT,which can accelerate migration. Celecoxib can inhibit this effect. So,COX2 may serve as a potential target for curing ovarian cancer.关键词
卵巢癌/COX2/增殖/转移/EMTKey words
Ovarian cancer/COX2/Proliferation/Metastasis/EMT分类
医药卫生引用本文复制引用
邓琳,冯定庆,凌斌..COX2促进卵巢癌细胞迁移及相关机制的研究[J].现代妇产科进展,2016,25(8):561-565,5.基金项目
国家自然科学基金资助项目(No:81372777,81372779) （No:81372777,81372779）
