现代妇产科进展2016，Vol.25Issue(8)：566-570,5.DOI:10.13283/j.cnki.xdfckjz.2016.08.002
Hsa-miR-6841-3 p对宫颈癌细胞系Caski增殖及侵袭转移的影响
Effect of miRNA-6841-3p on proliferation, migration and invasion of human cervical cancer cells
摘要
Abstract
Objective:To explore the effect of microRNA-6841-3p ( miRNA-6841-3p) on the biological behavior of human cervical cancer cells. Methods:The cervical cancer cell line Caski was transiently transfected with miRNA-6841-3 p mimics and miRNA-6841-3 p inhibi-tor by riboFECT CP. The expression of trefoil factor 3 (TFF3) and miRNA-6841-3p in human cervical cancer cell line Caski were detected by real-time fluorescent quantitative polymerase chain reaction (RT-PCR). Cell proliferation was evaluated using the cell counting Kit-8(CCK-8) assay. Invasion was measured by transwell chamber assays. The wound healing model was used to represent the migrationability. Results:The CaSki cells transfected with miRNA. miR-NA-6841-3 p mRNA expression in cells transfected with miRNA-6841-3 p mimics was signifi-cantly higher than that in negative control cells(P<0. 01). miRNA-6841-3p mRNA expression in cells transfected with miRNA-6841-3 p inhibitor was significantly lower than that in negative control cells (P<0. 01). However,the TFF3 mRNA expression in the mimics transfected cells decreased significantly. The overexpression of miRNA-6841-3p inhibited the viability,and inva-sion and migration abilities,as shown in the cells transfected with the miRNA-6841-3p mimics ( P<0 . 05 ) . Conclusion:Overexpression of miRNA-6841-3 p had an inhibitory effect on cell proliferation,and markedly inhibited invasion and migration of Caskicells in vitro and in vivo.关键词
Hsa-miR-6841-3 p/宫颈癌/Caski细胞/增殖/侵袭/转移Key words
Hsa-miR-6841-3 p/Cervical cancer/CaSki cell/Proliferation/Migration/Invasion分类
医药卫生引用本文复制引用
裴桂华,殷复粉,王宁,孙欣,于啸,孙业武,于风胜,王言奎..Hsa-miR-6841-3 p对宫颈癌细胞系Caski增殖及侵袭转移的影响[J].现代妇产科进展,2016,25(8):566-570,5.基金项目
国家自然科学基金资助项目(No:81172480) （No:81172480）
