现代妇产科进展2016，Vol.25Issue(8)：571-574,577,5.DOI:10.13283/j.cnki.xdfckjz.2016.08.003
沉默β-catenin对卵巢癌干细胞特性的影响
The effect to the cancer stem cell properties and cell proliferation of silencing β-catenin ;gene in ovarian cancer cell line SKOV3
摘要
Abstract
Objective:To explore the effect of the expression of β-catenin to ovarian cancer stem cell properties and cell proliferation. Methods:Byβ-catenin siRNA technology theβ-catenin gene of ovarian cancer cell line SKOV3 was knocked down, which named SKOV3-RNAi cells. The control group SKOV3 cells were transfected with control empty vector siRNA, which named SKOV3-NC cells. Both of these two groups of cells were cultured in serum-free suspension culture. The expressions of CD44 and CD117 were measured by flow cytometry, which was used to analyze the effect of silencing β-catenin gene of SKOV3 to the cancer stem cell properties of ovarian cancer cells. Plate cloning experiments,was used to count the number of cell formation after disposed by carboplatin. Detecting the cell growth by MTT method can re-flect the effect of silence β-catenin gene to cell proliferation. In vivo, the proliferation of the cells was detected by subcutaneous transplantation tumor in nude mice. Results:Compared with the control group SKOV3-NC cells,the cell spheres of SKOV3-RNAi cells in serum-free culture conditions were significantly reduced (P<0. 05),and the flow cytometry found the fluorescence intensity of CD44,CD117 of SKOV3-RNAi cell were lower than SKOV3-NC cells (P<0. 05). The plates clone assay showed that, after disposed to carboplatin, the proliferation of SKOV3-RNAi cells decreased more than SKOV3-NC cells as the number of cell colony formation was significant reduced. MTT assay found that,compared with the SKOV3-NC cells,the growth rate of SKOV3-RNAi cells slowed down. In vivo,SKOV3-RNAi tumorigenic ability much more de-creased than SKOV3-NC cells significantly. Conclusion:Silence the β-catenin gene of ovarian cancer cell line SKOV3 decreased the characteristics of stem cells significantly, and the cell proliferation is also inhibited.关键词
卵巢癌/β-catenin/肿瘤干细胞/细胞增殖Key words
Ovarian cancer/β-catenin/Cancer stem cells/Cell proliferation分类
医药卫生引用本文复制引用
张荷,张纳,袁蒙蒙,刘广芝..沉默β-catenin对卵巢癌干细胞特性的影响[J].现代妇产科进展,2016,25(8):571-574,577,5.基金项目
河南省医学科技攻关计划(No:201301007) （No:201301007）
