现代妇产科进展2016，Vol.25Issue(8)：586-588,592,4.DOI:10.13283/j.cnki.xdfckjz.2016.08.007
miR-617在宫颈癌组织中的表达及其对宫颈癌SiHa细胞生物学行为的影响
The expression of miR-617 in cervical cancer and the effects on cell biological behaviors ;in cervical carcinoma SiHa cell
李霞 1葛爱敏2
作者信息
- 1. 菏泽市立医院 妇科，菏泽 274000
- 2. 菏泽市立医院 病理科，菏泽 274000
- 折叠
摘要
Abstract
Objective:To investigate the expression of miR-617 in cervical cancer and the effects of its overexpression on cell biological behaviors such as proliferation,apoptosis and migration in cervical carcinoma SiHa cell. Methods:The expressions of miR-617 in cervical cancer and normal tissue were detected by real time RT-PCR,and miR-617 mimics were trans-fected into SiHa cell to make it overexpression, cell proliferation activity was determined by CCK-8 cell proliferation assay Kit,and cell apoptosis was detected by flow cytometry,flat cell cloning experiments was used toestimate cell colony-forming ability,cell migration were tested by wounding healing assay,and the expression of target genes c-Terminal binding protein 1 ( Ct-BP1) protein was determined by Western blot. Results:The expression of miR-617 in cervical cancer was down-regulated,and overexpression of miR-617 could inhibit SiHa cell proliferation, and promote cell apoptosis,and could inhibit SiHa cellclone formation as well as cell migration, overexpression of miR-617 downregulated the expression of protein CtBP1 . Conclusion:miR-617 in cervical cancer were down-regulated,and miR-617 can inhibit SiHa cell proliferation, clone formation and migration,and promote cell apoptosis,the mechanism may be relate to the downregulation of protein CtBP1 .关键词
miR-617/子宫颈癌/SiHa细胞/C-末端结合蛋白1Key words
miR-617/Cervical cancer/SiHa cell/C-terminal binding proteinl分类
医药卫生引用本文复制引用
李霞,葛爱敏..miR-617在宫颈癌组织中的表达及其对宫颈癌SiHa细胞生物学行为的影响[J].现代妇产科进展,2016,25(8):586-588,592,4.
