中国组织工程研究2016，Vol.20Issue(32)：4758-4763,6.DOI:10.3969/j.issn.2095-4344.2016.32.007
CD44+CD24-/low乳腺癌干细胞活性与多药耐药的相关性
Relationship between cell activity and multidrug resistance of CD44+CD24-/lowbreast cancer stem cells
翟晓建 1张浩 1张旖旎 2倪鸣 3王征1
作者信息
- 1. 南阳市中心医院乳腺甲状腺外科，河南省南阳市 473003
- 2. 南昌大学江西医学院，江西省南昌市 330000
- 3. 南阳市中心医院妇三科，河南省南阳市 473003
- 折叠
摘要
Abstract
BACKGROUND:Tumor stem cels are found to be involved in the recurrence, metastasis and drug resistance of the tumor. <br> OBJECTIVE:To explore the relationship between cel activity and multidrug resistance of CD44+CD24-/low breast cancer stem cels. <br> METHODS:CD44+CD24-/low breast cancer stem cels sorted from multidrug resistant breast cancer cel line MCF-7/ADR were detected as percentage using flow cytometry. P-gp fluorescence intensity of the cel membrane and MDR mRNA expression in sorted cels and MCF-7/ADR were detected using flow cytometry and RT-PCR, respectively. <br> RESULTS AND CONCLUSION: After sorting by flow cytometry, the proportion of CD44+CD24-/low breast cancer stem cels was more than 90%, indicating that the sorted cels could meet the needs of the subsequent experiment. CD44+CD24-/low cel subsets exhibited stronger ability to form microspheres than non- CD44+CD24-/low cel subsets. The P-gp fluorescence intensity and MDR mRNA expression of CD44+CD24-/low cels were significantly higher than those of MFC-7/ADR cel line (P < 0.05). These experimental findings suggest that CD44+CD24-/low breast cancer stem cels sorted from MCF-7/ADR cel lines have a strong ability to form cel microspheresin vitro, and significantly raise the level of P-gp protein and MDR mRNA expression, which may be one of the causes of multidrug resistance.关键词
干细胞/肿瘤干细胞/乳腺癌/细胞活性/细胞分选/多药耐药/体外成球实验/耐药基因分类
医药卫生引用本文复制引用
翟晓建,张浩,张旖旎,倪鸣,王征..CD44+CD24-/low乳腺癌干细胞活性与多药耐药的相关性[J].中国组织工程研究,2016,20(32):4758-4763,6.
