中国组织工程研究2016，Vol.20Issue(32)：4778-4784,7.DOI:10.3969/j.issn.2095-4344.2016.32.010
洛伐他汀对胶质瘤干细胞增殖和凋亡的影响
Effect of lovastatin on proliferation and apoptosis of glioma stem cells
闻公灵 1温昌明 1王彦平 1康梅娟 1周静 1张保朝1
作者信息
- 1. 南阳市中心医院神经内科，河南省南阳市 473009
- 折叠
摘要
Abstract
BACKGROUND:Increasing evidence has shown that lovastatin with less toxicity to normal cels has crucial effects on proliferation, apoptosis and differentiation of various cancer cels. However, its roles in glioma stem cels remain unclear. <br> OBJECTIVE:To explore the effect of lovastatin on proliferation and apoptosis of glioma stem cels. <br> METHODS: Flow cytometric sorting was used to separate glioma stem cels from human glioblastoma cel line U87. Effects of lovastatin on the proliferation and apoptosis of glioma stem cels were determined by MTT and flow cytometry, respectively. Furthermore, expression levels of Ki67, Bax and Bcl-2 in glioma stem cels treated with lovastatin were detected using western blot analysis. <br> RESULTS AND CONCLUSION: The CD133-positive glioma stem cels were sorted from human glioblastoma cel line U87 with a positive percentage of 85%. MTT assay showed that lovastatin inhibited the proliferation of glioma stem cels in dose (5, 10, 20 μmol/L)- and time (24, 48, 72, 96 hours)-dependent manners. Flow cytometry analysis showed that 10 μmol/L lovastatin (48 hours) induced apoptosis in glioma stem cels. In addition, the expression level of Ki67 was decreased by lovastatin treatment in a dose-dependent manner, and the Bcl-2 and Bax expression levels were reduced and increased by 10 μmol/L lovastatin treatment, respectively. In conclusion, lovastatin can inhibit cel proliferation and induce apoptosis of glioma stem cels, and lovastatin may be a potential drug for treatment of brain tumors.关键词
干细胞/肿瘤干细胞/胶质瘤干细胞/他汀类药物/洛伐他汀/CD133+胶质瘤细胞/增殖/凋亡分类
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闻公灵,温昌明,王彦平,康梅娟,周静,张保朝..洛伐他汀对胶质瘤干细胞增殖和凋亡的影响[J].中国组织工程研究,2016,20(32):4778-4784,7.
