中国组织工程研究2016，Vol.20Issue(33)：4869-4875,7.DOI:10.3969/j.issn.2095-4344.2016.33.001
雌激素对体外骨髓间充质干细胞成骨及成纤维相关基因的影响
Effects of estrogen on osteogenesis and fibroblast-related gene expression of bone marrow mesenchymal stem cells in vitro
摘要
Abstract
BACKGROUND:Bone marrow mesenchymal stem cel s have been shown to be differentiated into periodontal ligament fibroblasts when co-cultured with periodontal ligament cel s. Existing studies have shown that estrogen has the ability to influence bone marrow regeneration. OBJECTIVE:To investigate the effects of estrogen on osteogenesis and fibroblast-related factors alkaline phosphatase, type I and III col agen in bone marrow mesenchymal stem cel s. METHODS:Bone marrow mesenchymal stem cel s isolated from Beagle dogs were treated with estrogen. Osteogenesis and fibroblast-related mRNA and protein expression of bone marrow mesenchymal stem cel s was determined by RT-PCR and western blot assay, respectively. RESULTS AND CONCLUSION:mRNA and protein expression of type I and III col agen in bone marrow mesenchymal stem cel s was upregulated fol owing estrogen treatment;especial y, in contrast with type III col agen, the changes of type I col agen were more obvious. Estrogen did not influence mRNA and protein expression of alkaline phosphatase. These findings suggest that estrogen promotes the differentiation of bone marrow mesenchymal stem cel s into fibroblasts, whereas does not impact the genes involved in parodontium mineralization.关键词
组织构建/组织工程/雌激素/骨髓干细胞/碱性磷酸酶/胶原蛋白/国家自然科学基金分类
医药卫生引用本文复制引用
彭伟,秦媛,李坤河,陈松龄..雌激素对体外骨髓间充质干细胞成骨及成纤维相关基因的影响[J].中国组织工程研究,2016,20(33):4869-4875,7.基金项目
国家自然科学基金(81371111)；广东省自然科学基金(2014A030313059)@@@@the National Natural Science Foundation of China, No.81371111 （81371111）
the Natural Science Foundation of Guangdong Province, China, No.2014A030313059 （）
