现代医药卫生2016，Vol.32Issue(16)：2453-2455,3.DOI:10.3969/j.issn.1009-5519.2016.16.001
免疫磁珠法分离纯化乳鼠肾血管周细胞
Isolation and purification of sucking mice pericytes by immunomagnetic bead techniques
摘要
Abstract
Objective To establish the primary culture by renal cells and to investigate the isolation and purification method of sucking mice renal vessel pericytes by the imunomagnetic bead technique. Methods The kidney of healthy BALB/c sucking mice was selected,cut into pieces,digested and filtered for preparing the cellular suspension. The primary culture and passage(3 generations)were performed;the mimunomagnetic bead technique isolated neurogliocytes(NG-2);the inverted phase contrast microscope was adopted to observe the cellular morphological characteristics ,the immunohistochemistry was used to detect NG-2,SMA and CD31 expression by joint judgement,the cellular proliferation activity was detected by using the BrdU method. Whether meeting pericytes characteristics and whether purified cells having the proliferation activity were identified. Results The cells obtained by purification were wide and flat,the morphologies were fusiform, triangle or polygon with prominence. The cellular nucleus was ellipse and in the middle,the majority were mononucleus,occasionally bi-neucleus,with plentiful cytoplas-ma,without contacting inhibition growth,which conformed to the pericytes morphology and growth characteristics. NG-2 and SMA were positively expressed by immunocytochemical staining,CD31 was negatively expressed,which conformed to the pericytes characteristics. The 48,72 h proliferation activities of isolated and purified cells were (0.37±0.11)%and (0.42±0.10)%respec-tively. Conclusion The imunomagnetic bead method can make the pericytes to be isolated from the kidney with complex cellular compositions,which have biological activity,can continuously culture in vitro and provide the reference basis for subsequent study.关键词
免疫磁珠法/周细胞/肾小管/分离和提纯Key words
Immunobead method/Pericytes/Kidney tubules/Isolation and purification引用本文复制引用
王丽,马跃荣..免疫磁珠法分离纯化乳鼠肾血管周细胞[J].现代医药卫生,2016,32(16):2453-2455,3.基金项目
四川省卫生厅科技资助项目（80173）。 （）
