新医学2016，Vol.47Issue(8)：510-514,5.DOI:10.3969/j.issn.0253-9802.2016.08.003
组蛋白去乙酰化酶抑制剂对胶质瘤细胞增殖及 E2F1表达的影响
Effect of histone deacetylase inhibitor on the proliferation of glioma cells and expression of E2F1
摘要
Abstract
Objective To investigate the proliferation effect of histone deacetylase inhibitors (HDA-CIs)on the proliferation of glioma cells and expression of E2F1.Methods The glioma cell line U251 were transfected with two plasmids of she2f1a or she2f1b,empty vector BS /U6 was regarded as a control,the cell proliferation rate was detected by tetrazoliumsalt (MTT)assays and nuclear staining method.U251 cells were treated with TSA for 2 h,4 h and 8 h and treated with kinds of HDACIs including TAS,SAHA,M344,NaBu, VPA for 8 h.Dimethyl sulfoxide (DMSO)treatment was as control.Western blot was performed to test E2F1 expression.The glioma cell line U251 was treated with TSA for 8 h,12 h and 24 h,DMSO treatment was re-garded as control,and the cell proliferation rate was analyzed by MTT assays.U251 cells were treated with kinds of HDACIs including TSA,SAHA,M344,NaBu and VPA for 24 h,and then MTT assays were per-formed to determine cell proliferation rate.Results Compared with BS /U6,both she2f1a and she2f1b plas-mids can silence E2F1 expression and inhibit cell proliferation (P <0.05).Compared with control,no signifi-cant inhibition of E2F1 expression was found in TSA treated cells at 2 h and 4 h,and the E2F1 expression was significantly inhibited at 8 h with TSA treatment,and the other HDACIs can significantly suppress E2F1 ex-pression as well.MTT assays showed that TSA treatment 8 h samples did not remarkably affected cell prolifera-tion rate (P >0.05),however,TSA treatment 12 h samples significantly inhibited cell proliferation.(P <0.05)and induced more suppression effects when lasting up to 24 h (P <0.05).Moreover,the other HDACIs can significantly inhibit cell proliferation (P <0.05).Conclusion HDACIs can suppress E2F1 expression of glioma cell and cell proliferation meditated by E2F1.Result.关键词
神经胶质瘤/E2F1/组蛋白去乙酰化酶抑制剂Key words
Glioma/E2F1/Histone deacetylase inhibitors引用本文复制引用
袁忠民,梁琳,唐晓梅..组蛋白去乙酰化酶抑制剂对胶质瘤细胞增殖及 E2F1表达的影响[J].新医学,2016,47(8):510-514,5.基金项目
广东省自然科学基金（2015A030313475）；广州市科信局基金 （）
