烟草科技2016，Vol.49Issue(8)：1-7,7.DOI:10.16135/j.issn1002-0861.2015.0513
烟草谷胱甘肽转移酶基因NtGSTU17的鉴定及表达特性分析
Identification and expression analysis of a tobacco glutathione transferase gene NtGSTU17
摘要
Abstract
To understand the function of tobacco glutathione transferases, a tobacco GST of tau class, previously known as NtParC, was identified by cluster analysis, and its protein activity was analyzed in vitro as well. The gene expression pattern under the treatment of topping, paraquat and mancozeb, was also investigated by qRT-PCR. The results showed that the tau class gene, renamed as NtGSTU17, was 666 bp in length, encoding 221 amino acids, which exhibited higher CDNB conjugation activity. NtGSTU17 was constitutively expressed in tobacco, while the expression level in root was a little bit lower than the level in aerial part of tobacco. Topping could increase NtGSTU17 expression. Mancozeb spraying significantly up-regulated the expression level of NtGSTU17 in a quick way both in root and leaf, in comparison with the significantly down-regulation effect of paraquat on the root. Therefore, NtGSTU17 might be involved in the tobacco stress response, and showed selectivity to different herbicides.关键词
烟草/谷胱甘肽转移酶/NtGSTU17/CDNB偶联活性/表达模式/打顶/代森锰锌/百草枯Key words
Tobacco/Glutathione transferase/NtGSTU17/CDNB conjugation activity/Expression pattern/Topping/Mancozeb/Paraquat分类
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翟妞,刘萍萍,陈霞,李锋,毛李鸿,郑庆霞,武明珠,周会娜..烟草谷胱甘肽转移酶基因NtGSTU17的鉴定及表达特性分析[J].烟草科技,2016,49(8):1-7,7.基金项目
郑州烟草研究院院长科技发展基金项目“烟草谷胱甘肽转移酶家族（GSTs）一个tau类蛋白的晶体结构与功能研究”（902012CA0110）。 （）
