烟草科技2016，Vol.49Issue(8)：8-13,6.DOI:10.16135/j.issn1002-0861.2015.0145
毕节烤烟旺长期叶片代谢相关基因的差异表达及对气候的响应
Differential expression of genes related to flue-cured leaf metabolism and response to climate by tobacco varieties at fast growing stage in Bijie
摘要
Abstract
To investigate the effects of different tobacco varieties and climate on the expression of genes in pigment, nitrogen, starch and sugar metabolism pathway of flue-cured tobacco at mature stage, RT-PCR was used to analyze the gene expression in tobacco leaves of cv. Bina 1 and Jiucaiping 2 planted in Bijie(Guizhou) and Baofeng (Henan). The results showed that the expression levels of zeta-carotene desaturase (ZDS), chlorophyllase (CLH), glutamine synthetase (Gln1-3), nitrite reductase 3 (Nir-3), hexokinase (Hex), sucrose-6-phosphate phosphatase(SPP2)and trehalose-phosphate phosphatase(TPP)in Jiucaiping 2 planted in Bijie were higher than those in Bina 1 planted in Bijie. The results suggested that there were differences in metabolism pathway between Bina 1 and Jiucaiping 2. Nir-3 and cellulose synthase-like protein (CslE) gene expression in Bina 1 planted in Bijie expressed at a lower level and a higher level than those planted in Baofeng, respectively. The expression levels of Hex in Bina 1 and Jiucaiping 2 planted in Baofeng were higher than those planted in Bijie, and the expression of TPP in Jiucaiping 2 was lower than that planted in Bijie. This study indicated that these four genes(Nir-3, CslE, Hex and TPP)were affected mostly by climatic conditions.关键词
毕节地区/烤烟/旺长期/代谢/基因表达/气候响应Key words
Bijie/Flue-cured tobacco/Fast growing stage/Metabolism/Gene expression/Climatic response分类
农业科技引用本文复制引用
黄化刚,申燕,喻奇伟,危月辉,杨惠娟,史宏志..毕节烤烟旺长期叶片代谢相关基因的差异表达及对气候的响应[J].烟草科技,2016,49(8):8-13,6.基金项目
贵州省烟草公司毕节市公司科技项目“毕节特色烟标志蛋白的鉴定及分子标记开发研究”（BJYC-201302）；“促进毕节烟叶成熟期色素降解技术研究与应用”（BJYC-201305）。 （）
