烟草科技2016，Vol.49Issue(8)：14-20,7.DOI:10.16135/j.issn1002-0861.2015.0528
干旱胁迫下嫁接对烟草抗氧化酶活性、膜脂过氧化及胁迫响应基因表达的影响
Effects of grafting on antioxidant enzyme activities, membrane lipid peroxidation and stress-responsive gene expression in tobacco under drought stress
摘要
Abstract
To investigate the physiology and molecular mechanism of grafted tobacco in response to drought, taking drought-tolerant cultivar Longjiang 851 (LJ851) as a rootstock and drought-sensitive cultivar Jinxing 6007 as a scion, self-grafted (JX6007/JX6007) and rootstock-grafted (JX6007/LJ851) tobacco plants were prepared. A pot culture experiment was carried out to study the effects of grafting on the biomass, antioxidant enzyme activities, H2O2 and membrane lipid peroxidation in tobacco under drought stress. The results showed that under drought stress, the biomass, chlorophyll contents and antioxidant enzyme activities of JX6007/LJ851 were higher than those of JX6007/JX6007 by different extents, while the membrane lipid peroxidation degree and H2O2 content were lower than those of JX6007/JX6007. qRT-PCR indicated that the expressions of stress-responsive genes (LEA5, AREB, CDPK2 and ERD10C) in rootstock-grafted plants were significantly up-regulated. Therefore, grafted tobacco with drought-tolerant rootstock genotype might improve drought tolerance in tobacco via regulating antioxidant enzyme activities and stress-responsive gene expression.关键词
烟草/干旱胁迫/嫁接/抗氧化酶/膜脂过氧化/基因表达Key words
Tobacco/Drought stress/Grafting/Antioxidant enzyme/Membrane lipid peroxidation/Gene expression分类
农业科技引用本文复制引用
霍勇锦,徐紫薇,王燃,王欢颜,刘剑君,苏新宏,张威,杨铁钊,夏宗良..干旱胁迫下嫁接对烟草抗氧化酶活性、膜脂过氧化及胁迫响应基因表达的影响[J].烟草科技,2016,49(8):14-20,7.基金项目
河南省烟草公司重点科技项目“利用嫁接提高烤烟抗旱性的关键技术研究”（HYKJ201202）。 （）
