烟草科技2016，Vol.49Issue(8)：51-56,6.DOI:10.16135/j.issn1002-0861.2016.0053
基于两种细胞的卷烟烟气体外免疫毒性评价
Evaluation of in vitro immunotoxicity of mainstream cigarette smoke by using two kinds of cells
摘要
Abstract
To establish the in vitro immunotoxicity of cigarette smoke, mouse lymphoma cell EL-4 and mouse macrophage cell Ana-1 were treated with the total particular matter (TPM) in mainstream smoke of Kentucky 3R4F reference cigarette, then the viability of cells was measured by CCK-8 assay. Luminex liquid chip was used to determine the levels of 20 cytokines (FGF-basic, GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17, KC, IP-10, MCP-1, MIP-1α, VEGF, MIG, TNF-α) in the supernatant. The results showed that after exposure to TPM, GM-CSF, IL-10, VEGF were inhibited in EL-4, while MCP-1, MIP-1α, TNF-α were induced in Ana-1. The levels of the other 17 cytokines in two cell types did not change significantly with the increase of TPM concentration. The cell reactions of different types or sources to the toxicological effect of TPM of cigarette smoke differed, therefore adopting two kinds of cells is helpful to the better evaluation of in vitro immunotoxicity of cigarette smoke. The method is rapid, sensitive, high-throughput and suitable for assessing in vitro immunotoxicity of cigarette smoke.关键词
Luminex液相芯片/卷烟烟气/总粒相物/EL-4细胞/Ana-1细胞/体外免疫毒性Key words
Luminex liquid chip/Cigarette smoke/Total particular matter (TPM)/EL-4 cell/Ana-1 cell/In vitro immunotoxicity分类
轻工纺织引用本文复制引用
赵俊伟,杨陟华,李翔,谢复炜,潘秀颉,朱茂祥,刘惠民,谢剑平..基于两种细胞的卷烟烟气体外免疫毒性评价[J].烟草科技,2016,49(8):51-56,6.基金项目
中国烟草总公司郑州烟草研究院院长科技发展基金项目“基于Luminex技术的卷烟烟气体外免疫毒性检测方法及应用”（322014CA0350）；中国烟草总公司郑州烟草研究院科技项目“基于MRTP健康风险评估框架的体外毒理学测试方法研究”(322013CZ0600）；国家科技支撑计划项目“食品中热反应伴生危害物毒理学检测及风险评估关键技术研究”（2012BAK01B03）。 （）
