针刺研究2016，Vol.41Issue(4)：283-290,8.DOI:10.13702/j.1000-0607.2016.04.001
耳穴区经皮电刺激对颞叶癫痫大鼠癫痫发作频率及海马区白介素-1β、肿瘤坏死因子-α表达的影响
Effects of Transcutaneous Electrostimulation of Auricular Points on Behavior and Hippocampal IL-1 βand TNF-αExpression in Temporal Lobe Epilepsy Rats
摘要
Abstract
Objective To observe the effect of transcutaneous otopoint electrostimulation (TCOES)on behavior and ex-pression of hippocampal interleukin-1 β (IL-1 β)and tumor necrosis factor-α (TNF-α)expression in lithium-pilocarpine induced chronic spontaneous temporal lobe epilepsy (TLE)rats,so as to investigate its antiepileptic mechanism.Methods Thirty-six SD rats were randomly divided into control,model and TCOES groups (n = 1 2 in each group).The epilepsy model was estab-lished by intraperitoneal injection of lithium chloride (1 27.2 mg/kg),scopolamine(1 mg/kg,20 h after the 1 st injection)and pilo-carpine (1 0 mg/kg,30 min after scopolamine injection).Rats of the control group were treated by injection of normal saline (i.p.i.). Transcutaneous electrostimulation (1 mA,20 Hz)was applied to bilateral otopoints “Heart”“Lung”and “Subcortex”for 20 min, once daily for 6 weeks except the weekends.The behavior reactions were observed by a video monitoring system.The expres-sion of IL-1 βand TNF-αproteins and genes in the hippocampus were determined by immunofluorescence and quantitative real-time PCR,separately.Results Behavioral observation showed that after TCOES intervention,the frequency of epilepsy attack was significantly decreased in comparison with pre-treatment (P <0.05).Immunofluorescence and real-time PCR showed that com-pared with the control group,the immunoactivity of IL-1 βand TNF-α in both hippocampal CA 1 and CA 3 regions and hippocampal IL-1 βand TNF-αgene expression were obviously increased in the model group (P <0.05,P <0.0 1 ).Following TCOES,the in-creased hippocampal IL-1 β and TNF-α and IL-1 β mRNA and TNF-α mRNA expression levels were all suppressed (P <0.05, P <0.0 1 ).Conclusion TCOES intervention has an antiepileptogenic effect in temporal lobe epilepsy rats,which may be relat-ed to its effects in down-regulating expression of proinflammatory cytokine IL-1 βand TNF-α in the hippocampus.关键词
颞叶癫痫/经皮耳穴电刺激/海马/白介素-1 β/肿瘤坏死因子-αKey words
Temporal lobe epilepsy/Transcutaneous otopoint electrical stimulation/Hippocampus/IL-1 β/TNF-α分类
医药卫生引用本文复制引用
杨海龙,乔丽娜,谭连红,杨娇娇,陈忠,张永臣,杨永升..耳穴区经皮电刺激对颞叶癫痫大鼠癫痫发作频率及海马区白介素-1β、肿瘤坏死因子-α表达的影响[J].针刺研究,2016,41(4):283-290,8.基金项目
项目来源:国家自然科学基金面上项目(No.81273833) （No.81273833）
国家自然科学基金青年科学基金项目(No.81303055) （No.81303055）
