针刺研究2016，Vol.41Issue(4)：291-297,7.DOI:10.13702/j.1000-0607.2016.04.002
电针与艾灸对内脏高敏感大鼠穴区辣椒素受体和热休克蛋白70表达的影响及镇痛效应
Effect of Electroacupuncture and Moxibustion on Visceral Pain and Expression of VR 1 and HSP 70 of “Tianshu”(ST 25)Region in Colorectal Distension-induced Visceral Hypersensitivity Rats
摘要
Abstract
Objective To compare the effects of electroacupuncture (EA)and moxibustion (Moxi)on visceral pain and expression of vanilloid receptor subtype 1 (VR 1 )and heat shock protein (HSP)70 in “Tianshu”(ST 25)region in colorectal dis-tension (CRD)-induced visceral hypersensitivity (VHS)rats.Methods Fifty male SD rats were randomly divided into normal control,VHS model,43 ℃-moxi,46 ℃-moxi,1 mA-EA and 3 mA-EA groups (n = 1 0 in each group).The VSH model was estab-lished by CRD once daily for 1 4 days.EA or Moxi stimulation was applied to bilateral “Tianshu”(ST 25)for 1 0 min,once daily for consecutive 1 0 days.The abdominal withdrawal reflex (AWR)scores (0-4 points)were rated according to Al-Chaer’s and co-workers’standards (2000)and the expression levels of VR 1 and HSP 70 in bilateral ST 25 area tissues detected by immunohisto-chemistry.Results The AWR scores for 20,40,60 and 80 mmHg CRD pressures were significantly increased compared to the normal control group (P <0.0 1 )and notably decreased after 43 ℃-and 46 ℃-moxi,and 1 mA-and 3 mA-EA stimulation of bila-teral ST 25 in comparison with the model group (P <0.05,P <0.0 1 ),and the effect of 46 ℃-moxi was apparently superior to those of 1 mA-EA at 40 and 80 mmHg,and 3 mA-EA at 40 mmHg (P <0.05).After modeling,the expression of both VR 1 and HSP 70 (percentages of area of positive-cells)in ST 25 region had no significant changes (P >0.05).Compared to the model group,the expression levels of VR 1 in the 43 ℃-moxi and 46 ℃-moxi groups,and HSP 70 in the 43 ℃-moxi and 46 ℃-moxi, 1 mA-EA and 3 mA-EA groups were significantly up-regulated (P <0.0 1 ),but without obvious changes in the expression of VR 1 in the 1 mA-EA and 3 mA-EA groups (P >0.05).The effects of 46 ℃-moxi were considerably better than those of 43 ℃-moxi, 1 mA-EA and 3 mA-EA in up-regulating VR 1 and HSP 70 expression (P <0.05,P <0.0 1 ).No significant differences were found among the 43 ℃-moxi,1 mA-EA and 3 mA-EA groups in the expression of VR 1 and HSP 70 (P >0.05).Conclusion Moxibus-tion at 43 ℃ and 46 ℃ and EA at 1 mA and 3 mA,especially the 46 ℃-moxi,can relieve visceral pain in visceral hypersensitivity rats,which may be related to their effects in up-regulating expression of VR 1 and HSP 70 in “Tianshu”(ST 25)area.关键词
内脏高敏感/电针/艾灸/天枢穴区/辣椒素受体 1/热休克蛋白 70Key words
Visceral hypersensitivity/Electroacupuncture/Moxibustion/Tianshu (ST 25)area/Vanilloid recep-tor-1/Heat shock protein-70分类
医药卫生引用本文复制引用
李雨薇,赵继梦,陈柳,商海霞,吴璐一,包春辉,窦传字,吴焕淦,施茵..电针与艾灸对内脏高敏感大鼠穴区辣椒素受体和热休克蛋白70表达的影响及镇痛效应[J].针刺研究,2016,41(4):291-297,7.基金项目
项目来源:国家重点基础研究发展计划(“973”计划,No.2015 CB 554500) （“973”计划,No.2015 CB 554500）
国家自然科学基金(No.30973784) （No.30973784）
