针刺研究2016，Vol.41Issue(4)：298-302,5.DOI:10.13702/j.1000-0607.2016.04.003
P 物质阳性表达神经纤维在大鼠心包经五输穴局部皮肤组织中的分布
Distribution of Substance P-positive Nerve Fibers in Local Skin Tissues of Five Shu-points of the Pericardium Meridian in the Rat
摘要
Abstract
Objective To investigate the distribution of substance P (SP)-positive nerve fibers in local skin tissues of the five shu-points of the Pericardium Meridian in rats for revealing the differential innervations of the five acupoints.Methods Six adult male SD rats were used in the present study.Under deep anesthesia,the rats received transcardiac perfusion with 4%polyformaldehyde+phosphate buffered solution,followed by dissecting the local skin tissues from the sites corresponding to five shu-points “Zhongchong”(PC 9),“Laogong”(PC 8),“Daling”(PC 7),“Jianshi”(PC 5),and “Quze”(PC 3)in the human body.The tissues were sagittally cut into sections (20 μm)using a freezing microtome,followed by fluorescent immunohistochemi-cal and histochemical staining with SP,phalloidin and DAPI,respectively.The distribution of SP-positive fibers were observed under fluorescent microscope and laser confocal microscope.Results In general,SP expressed on the thin sensory fibers in the skin tissue.SP-positive nerve fibers distributed in a free single style in the epidermis and dermis,and gathered together to form a bundle in the subcutaneous layer.After counting the number and length of free single fibers in the epidermis and dermis of each of the five shu-points,we found that no significant differences existed in the density between the PC 9 and PC 8 regions,as well as among the PC 7,PC 5,and PC 3 regions (P >0.05).In contrast,the density of SP-positive nerve fibers in the former two acu-points (PC 9 and PC 8)was significantly higher than that of anyone of the latter three acupoints (P <0.0 1 ).In addition,SP posi-tive nerve fibers mainly ran in parallel to the vessel-like structure.Conclusion The local skin tissues of acupoint PC 9,PC 8, PC 7,PC 5,and PC 3 of the Pericardium Meridian express SP-positive fibers,which presents a decreasing tendency in the densi-ty from the distal to the proximal part of the forelimb,reflecting a relative specificity of the innervations of the five shu-points.关键词
五输穴/心包经/皮肤神经支配/P 物质/神经纤维/穴位特异性Key words
Five shu-points/Pericardium Meridian/Skin innervation/Substance P (SP)/Nerve fibers/Speci-ficity of acupoints分类
医药卫生引用本文复制引用
张知云,徐东升,崔晶晶,张坤,景向红,白万柱..P 物质阳性表达神经纤维在大鼠心包经五输穴局部皮肤组织中的分布[J].针刺研究,2016,41(4):298-302,5.基金项目
项目来源:国家自然科学基金资助项目(No.81373557、No.81403327) （No.81373557、No.81403327）
中国中医科学院针灸研究所自主选题项目(No.ZZ 08002) （No.ZZ 08002）
