针刺研究2016，Vol.41Issue(4)：303-307,333,6.DOI:10.13702/j.1000-0607.2016.04.004
热补针法对风寒湿阻型类风湿关节炎患者血浆中能量代谢相关基因 Atp 5O、Atp 6 V 1 B 2表达的影响
Influence of Heat-reinforcing Needling on Expression of Plasma Atp 5 O mRNA and Atp 6 V1B2 mRNA in Patients with Rheumatoid Arthritis of Wind-cold-damp Retention Type
摘要
Abstract
Objective To observe influences of heat-reinforcing needling (HRN)on scores of traditional Chinese medi-cine (TCM)symptoms and expression of plasma ATP synthase subunit O (Atp 5 O )mRNA and lysosomal V 1 subunit B 2 (Atp 6 V 1 B 2 )mRNA in patients with wind-cold-damp retention type rheumatoid arthritis (RA),so as to investigate its biological mech-anisms in “heat production”.Methods Sixty wind-cold-damp retention type RA patients were randomly allocated to HRN group (n =30)and control group (n =30).Guanyuan (CV 4),Qihai (CV 6),bilateral Zusanli (ST 36),and local acupoints near the knee-joint were selected for needling stimulation.Patients of the HRN group were treated by manipulating the acupuncture needle with HRN,and those of the control group treated by manipulating the needle with uniform reinforcing-reducing method.The treat-ments were performed once daily,5 days a week,and two weeks altogether.The other 30 healthy volunteers were recruited as the normal control group.The TCM symptom scoring system (0-3 1 points,1 1 items as the severities of pain,swelling and ten-derness of the knee-joint)was used to evaluate the status of RA,and quantitative real-time PCR (RT-PCR)was used to detect the expression of plasma Atp 5 O mRNA and Atp 6 V 1 B 2 mRNA following removal of red blood cells.Results After the treat-ment,the TCM scores of both the HRN and control groups were significantly decreased (P <0.05),and that of the HRN group was significantly lower than that of the control group (P <0.05).Before the treatment,the expression levels of plasma Atp 5 O mRNA and Atp 6 V 1 B 2 mRNA in RA patients were significantly lower than those of the normal group (P <0.05),and after the treatment,the expression levels of plasma Atp 5 O mRNA and Atp 6 V 1 B 2 mRNA were significantly increased in both HRN and control groups compared to pre-treatment in the same one group (P <0.05),and the up-regulated Atp 5 O mRNA and Atp 6 V 1 B 2 mRNA levels were remarkably higher in the control group than in the HRN group (P <0.05).Conclusion Both heat-produ-cing needling and uniform reinforcing-reducing needling can improve RA patients’clinical symptoms,which may be associated with their actions in up-regulating expressions of plasma Atp 5 O mRNA and Atp 6 V 1 B 2 mRNA.关键词
类风湿关节炎/手针/热补针法/ATP 合成酶亚单位 O/溶酶体 V 1 亚单位 B 2Key words
Rheumatoid arthritis/Manual acupuncture/Heat-producing needling/Plasma ATP synthase subunit O (Atp 5 O )mRNA/Lysosomal V 1 subunit B 2 (Atp 6 V 1 B 2 )mRNA分类
医药卫生引用本文复制引用
杜小正,王金海,张星华,田杰祥,秦晓光,方晓丽,田亮,袁博..热补针法对风寒湿阻型类风湿关节炎患者血浆中能量代谢相关基因 Atp 5O、Atp 6 V 1 B 2表达的影响[J].针刺研究,2016,41(4):303-307,333,6.基金项目
项目来源:甘肃省自然科学基金项目(No.1208 RJZA 185) （No.1208 RJZA 185）
国家中医药管理局甘肃郑氏针法学术流派传承工作室项目(No.2305135901) （No.2305135901）
