针刺研究2016，Vol.41Issue(4)：308-313,6.DOI:10.13702/j.1000-0607.2016.04.005
电针对不同时程焦虑样行为大鼠海马神经元型一氧化氮合酶的影响
Effects of Electroacupuncture on Hippocampal nNOS Expression in Rats with Anxiety-like Behavior
摘要
Abstract
Objective To compare the effects of electroacupuncture (EA)preconditioning and post-conditioning on hip-pocampal neuronal nitric oxide synthase (nNOS)expression in rats with anxiety-like behavior,so as to explore the reasonable EA intervention time.Methods Forty-two SD rats were randomly divided into 6 groups:normal 1 ,normal 2,anxiety model 1 ,an-xiety model 2,EA-pre-conditioning and EA-post-conditioning.The anxiety model was established by giving the rats with repeated foot shock stimulation (0.8 mA,2-25 s/time,1 0 times in 5 min)combined with isolation-raising.Before or after modeling,EA stimulation was applied to “Baihui”(GV 20)and “Yintang”(GV 29)for 20 min,once a day for 7 days.Elevated plus maze (EPM)tests were performed to determine the percentages of time spent in the open arms and the percentages of entries into the open arms in 5 min for evaluating the animals'anxiety-like behavioral activities.The reverse transcriptase polymerase chain reac-tion (RT-PCR)was used to detect hippocampal nNOS mRNA expression and the immunohistochemical staining adopted to detect the expression of nNOS protein in the hippocampal CA 1 and CA 3 areas,respectively.Results Compared with their own nor-mal control group 1 and 2,both the percentage of time spent in the open arms and the percentage of entries into the open arms were significantly decreased in model group 1 and 2 (P <0.05,P <0.0 1 ).After pre-and post-conditioning of EA,the decreased proportions of time spent and entries into the open arms were considerably increased (P <0.05),suggesting an improvement of anxiety-like behavior activities after EA intervention.The expression levels of hippocampal nNOS mRNA and nNOS protein in the hippocampal CA 3 region were significantly higher in the model group 1 and 2 than in their own normal control group 1 and 2 (P <0.0 1 ),but those of nNOS protein in the CA 1 area were markedly lower in the model group 1 and 2 than in their own normal control group 1 and 2 (P <0.0 1 ).Following pre-and post-conditioning of EA,the increased expression levels of nNOS mRNA and protein of CA 3 area and the decreased level of nNOS protein of CA 1 area were all notably reversed (P <0.05,P <0.0 1 ). Conclusion Both pre-and post-conditioning of EA can improve anxiety-like behavior in anxiety rats,which may be associated with its effects in down-regulating hippocampal nNOS expression.关键词
足底电击/焦虑/神经元型一氧化氮合酶/电针/高架十字迷宫Key words
Inevitable foot shock/Anxiety/Neuronal nitric oxide synthase(nNOS)/Electroacupuncture (EA)/Elevated plus maze(EPM)分类
医药卫生引用本文复制引用
汪婷婷,耿婷婷,姜劲峰,徐旺芳,荣长保..电针对不同时程焦虑样行为大鼠海马神经元型一氧化氮合酶的影响[J].针刺研究,2016,41(4):308-313,6.基金项目
项目来源:国家自然科学基金(No.81373747) （No.81373747）
