针刺研究2016，Vol.41Issue(4)：314-320,7.DOI:10.13702/j.1000-0607.2016.04.006
双侧电针结合康复训练对局灶性脑缺血/再灌注损伤大鼠海马 CA 3区神经生长相关蛋白43及突触素表达的影响
Effect of Electroacupuncture Combined with Rehabilitation Training on Neurological Function and Expression of Neuronal Growth Associated Protein 43 and Synaptophysin in Rats with Focal Cerebral Ischemia/Reperfusion Injury
卿鹏 1柴铁劬 2丁红梅 3赵仓焕 1胡静1
作者信息
- 1. 暨南大学附属第一医院针灸科，广州 510630
- 2. 广州中医药大学针灸康复临床医学院，广州 510405
- 3. 广州中医药大学第一附属医院重症医学科，广州 510405
- 折叠
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA)combined with rehabilitation training on motor function and expression of neuronal growth associated protein 43 (GAP-43)and synaptophysin (SYP)in hippocampal CA 3 region in rats with focal cerebral ischemia/reperfusion injury (CI/RI).Methods A total of 46 SD rats were randomized into normal control,CI/RI model,rehabilitation training (RT),paralysis-side (unilateral)-EA+RT,and bilateral-EA+RT groups (n =6 in the normal control group,and n = 1 0 in each of the other group).The CI/RI model was established by occlusion of the middle cerebral artery (MCAO)and reperfusion.EA (5 Hz/1 0 Hz,2 mA)was applied to the unilateral “Quchi”(LI 1 1 )and “Housanli”(ST 36) on the affected side or bilateral LI 1 1 and ST 36 for 30 min,once daily for two weeks except the Sunday.The neurological deficit severity (Zea Longa score)was assessed 24 h,7 and 1 4 days after modeling.The immunoactivity of GAP-43 and SYP in the CA 3 region of the hippocampus was detected using immunohistochemistry.Pathological changes of the prefrontal cortex was ob-served after H.E.staining.Results Following modeling,the neurological deficit scores of the model,RT,unilateral-EA+RT and bilateral-EA+RT groups were gradually decreased,and were significantly lower on day 7 and 1 4 in the bilateral-EA+ RT group and on day 1 4 in the unilateral-EA+RT group than in the model group (P <0.05).The effect of the bilateral-EA+RT group was obviously superior to those of both RT and unilateral EA+RT groups in improving neurological function (P <0.05).Results of immunohistochemical staining displayed that the expression levels of GAP-43 and SYP in the CA 3 region of hippocampus were sig-nificantly up-regulated in the model group than in the normal control group (P <0.05),and further obviously up-regulated in both unilateral-and bilateral-EA+RT groups (P <0.05).No significant changes of GAP-43 and SYP protein expression in the RT group compared with the model group (P >0.05),and the expression levels of GAP-43 and SYP protein in the bilateral-EA+RT were significantly higher than those in the unilateral EA+RT group (P <0.05).H.E.staining showed that the ischemic injury of cells (neuronal apoptosis and enlargement of intercellular space)of the prefrontal cortex was relatively milder in the EA+RT groups. Conclusion EA plus RT can promote the recovery of motor function in CI/RI rats,which may be associated with its function in increasing the expression of GAP-43 and SYP in hippocampal CA 3 region.The effects of bilateral-EA+RT is obviously better than those of unilateral EA+RT.关键词
局灶性脑缺血/再灌注损伤/电针/康复训练/神经生长相关蛋白 43/突触素/海马 CA 3 区/运动功能Key words
Cerebral ischemia-reperfusion injury (CI/RI)/Electroacupuncture/Rehabilitation training/Neuronal growth associated protein 43 (GAP-43)/Synaptophysin (SYP)/Hippocampal CA 3 region/Motor function分类
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卿鹏,柴铁劬,丁红梅,赵仓焕,胡静..双侧电针结合康复训练对局灶性脑缺血/再灌注损伤大鼠海马 CA 3区神经生长相关蛋白43及突触素表达的影响[J].针刺研究,2016,41(4):314-320,7.
