针刺研究2016，Vol.41Issue(4)：321-326,6.DOI:10.13702/j.1000-0607.2016.04.007
直接灸“肝俞”对原发性肝癌癌前病变大鼠 T 淋巴细胞亚群的影响
Effects of Direct Moxibustion of “Ganshu”(BL 18)on the Contents of T cells in Peripheral Blood in Rats with Precancerous Lesion of Primary Hepatocellular Carcinoma
摘要
Abstract
Objective To observe the effect of moxibustion stimulation of “Ganshu”(BL 1 8)region on contents of T cells in the peripheral blood in rats with Diethylnitrosamine (DEN)-induced hepatocellular carcinoma (HCC),so as to explore its effec-tive in improving immunoregulatory function.Methods Seventy male Wistar rats were randomly divided into control group (n = 1 0),model group(n = 1 5),direct moxibustion-1 5 s group(n = 1 5),direct moxibustion-30 s group (n = 1 5)and ginger-sepa-rated moxibustion group(n = 1 5).The primary HCC precancerous lesion model was established by intraperitoneal injection of DEN (50 mg/kg),once every 3 days for 1 0 weeks.Moxibustion was applied to bilateral “Ganshu”(BL 1 8)for about 1 5 min (3 moxa-cones),or 30 min (six moxa-cones),with or without ginger-slice separation,once every other day for 1 0 consecutive weeks. The contents of T cells of CD 3 + ,CD 4 + ,CD 8 + and CD 4 +/CD 8 + ratio in the peripheral blood were detected with Flow Cy-tometey(FCM),pathological changes of liver were observed by light microscope after hematoxylin-eosin(HE)stain.Results Compared to the control group,the contents of blood CD 3 + and CD 4 + T cells and ratio of CD 4 +/ CD 8 + were significantly down-regulated,while that of CD 8 + T cells was obviously increased in the model group(P <0.05,P <0.0 1 ).After moxibustion intervention,the decreased CD 3 + and CD 4 + T cells and CD 4 +/CD 8 + levels and the increased CD 8 + T cell contents were re-versed in all the 3 moxibustion groups (P <0.05, P <0.0 1 ),except CD 3 + in the ginger-separated moxibustion group (P >0.05).There were no significant differences among the three moxibustion groups in the CD 3 + ,CD 4 + ,CD 8 + and CD 4 +/CD 8 + levels (P >0.05).In addition,the pathological changes of liver tissue as central vein deviation or absence,disordered ar-rangement of hepatic cords,narrowing of the hepatic sinusoid,hyperplasia of collagen fibers,formation of tuberosis,unevenness of liver cells with nuclear anachromasis and higher heteromorphism,and macronucleus oncocytes in primary HCC rats were not ob-served or milder after moxibustion intervention.Conclusion Direct moxibustion and ginger-separated moxibustion can improve pathological changes of hepatic cells in rats with HCC,which may be associated with its actions in raising blood CD 3 + and CD 4 +T cell contents and reducing CD 8 + T levels to improve immune function.关键词
肝癌/直接灸/隔姜灸/癌前病变/T 淋巴细胞/肿瘤免疫Key words
Hepatocellular carcinoma (HCC)/Direct moxibustion/Ginger-separated moxibustion/Precancerous lesion/T cells/Tumor immunity分类
医药卫生引用本文复制引用
闫亚南,王宁,王卓昱,田瑶,成宇镇,马惠芳,侯中伟,马佳佳,关翰宇..直接灸“肝俞”对原发性肝癌癌前病变大鼠 T 淋巴细胞亚群的影响[J].针刺研究,2016,41(4):321-326,6.基金项目
项目来源:国家自然科学基金青年科学基金项目(No.81202760) （No.81202760）
