中国病理生理杂志2016，Vol.32Issue(8)：1345-1350,6.DOI:10.3969/j.issn.1000-4718.2016.08.001
MEF2 C 介导 microRNA－214发挥抑制心肌细胞肥大的作用
MEF2C mediates inhibitory effect of microRNA-214 on cardiomyocyte hypertrophy
摘要
Abstract
AIM:To investigate the effect of microRNA-214 ( miR-214) on cardiomyocyte hypertrophy and the expression of the potential target genes .METHODS:A cell model of hypertrophy was established based on angiotensin-Ⅱ( Ang-Ⅱ)-induced neonatal mouse ventricular cardiomyocytes ( NMVCs) .Dual luciferase reporter assay was performed to verify the interaction between miR-214 and the 3’ UTR of MEF2C.The expression of MEF2C and hypertrophy-related genes at mRNA and protein levels was determined by RT-qPCR and Western blot , respectively .RESULTS:The expression of ANP, ACTA1,β-MHC and miR-214 was markedly increased in Ang-Ⅱ-induced hypertrophic cardiomyocytes .Dual lu-ciferase reporter assay revealed that miR-214 interacted with the 3’ UTR of MEF2C, and miR-214 was verified to inhibit MEF2C expression at the transcriptional level .The protein expression of MEF2C was markedly increased in the hypertro-phic cardiomyocytes .Moreover, miR-214 mimic, in parallel to MEF2C siRNA, inhibited the expression of hypertrophy-re-lated genes in Ang-Ⅱ-induced NMVCs.CONCLUSION:MEF2C is a target gene of miR-214, which mediates the effect of miR-214 on attenuating cardiomyocyte hypertrophy .关键词
心肌肥厚/微小RNA-214/MEF2C/心肌细胞Key words
Cardiac hypertrophy/MicroRNA-214/MEF2C/Cardiomyocytes分类
医药卫生引用本文复制引用
唐春梅,吴书林,单志新,朱杰宁,朱文思,林秋雄,胡志琴,符永恒,张梦珍,邓春玉,谭虹虹..MEF2 C 介导 microRNA－214发挥抑制心肌细胞肥大的作用[J].中国病理生理杂志,2016,32(8):1345-1350,6.基金项目
国家自然科学基金资助项目（ No．81270222；No．81470439）；广东省自然科学基金资助项目（ No．2014A030313635）；广东省医学研究基金资助项目 （）
