中国病理生理杂志2016，Vol.32Issue(8)：1370-1375,6.DOI:10.3969/j.issn.1000-4718.2016.08.005
枸杞多糖通过调控 PI3 K／Akt／eNOS 信号通路对去卵巢大鼠心肌产生抗氧化作用
LBP regulates PI3 K/Akt/eNOS signaling pathways in ovariectomized rat myocardium to exert antioxidative effect
摘要
Abstract
AIM:To observe the influence of Lycium barbarum polysaccharide (LBP) on the PI3K/Akt/eNOS signaling pathways in ovariectomized rat myocardium .METHODS:Female SD rats (n=30) were divided into sham oper-ation group , ovariectomized group , progynova group , high-dose LBP group and low-dose LBP group .The serum levels of estradiol, lactate dehydrogenase (LDH) and creatine kinase (CK) were measured by ELISA.The myocardial contents of H2 S and oxidative stress injury-related indicators were also detected .The morphological changes of the myocardium were observed with HE staining.The expression of eNOS and PI3K/Akt pathway-related proteins in the myocardium was deter-mined by Western blot .RESULTS: Compared with sham operation group , the serum level of estradiol , the content of H2 S, the activity of GSH-Px, and the expression of eNOS and PI3K/Akt pathway-related proteins in the myocardium in ovariectomized group were all decreased , and the levels of ROS and MDA in the myocardium were increased (P<0.05). The serum levels of LDH and CK were also increased .The arrangement of the myocardial cells was disordered , and the in-tercellular space was also increased in the ovariectomized group .Compared with ovariectomized group , the serum level of estradiol, the myocardial levels of H2S and GSH-Px, and the protein levels of eNOS and phosphorylated Akt were all in-creased in high dose group, while the levels of ROS and MDA in the myocardium were decreased (P<0.05).The serum levels of LDH and CK were also decreased .The morphological changes of the rat myocardium were improved in high dose group.CONCLUSION: LBP prevents and treats postmenopausal cardiovascular lesions through regulating PI 3K/Akt/eNOS signaling pathways in ovariectomized rats .关键词
枸杞多糖/去卵巢大鼠/心血管疾病/PI3K/Akt/eNOS信号通路Key words
Lycium barbarum polysaccharides/Ovariectomized rats/Cardiovascular diseases/PI3K/Akt/eNOS signaling pathways分类
医药卫生引用本文复制引用
于宁,杨芳,冷雪,张妮,王俊岩,宋囡..枸杞多糖通过调控 PI3 K／Akt／eNOS 信号通路对去卵巢大鼠心肌产生抗氧化作用[J].中国病理生理杂志,2016,32(8):1370-1375,6.基金项目
辽宁中医药大学中医脏象理论及应用教育部重点实验室开放基金资助项目（No．F14－231－1－17）；辽宁中医药大学大学生创新创业训练计划 （）
