中国病理生理杂志2016，Vol.32Issue(8)：1484-1491,8.DOI:10.3969/j.issn.1000-4718.2016.08.025
两种慢性渗透性腹泻大鼠模型建立方法的比较
Comparison of 2 methods for establishing rat model of chronic osmotic diarrhea
摘要
Abstract
AIM:To compare 2 different rat models of chronic osmotic diarrhea with the lactose enrichment and magnesium citrate methods.METHODS:The SD rats (n=31) were randomly divided into lactose enrichment diar-rhea group (Lac group, 12 rats), magnesium citrate diarrhea group (Mg group, 12 rats) and normal control group (Con group, 7 rats).The rats in Lac group were fed with a lactose enrichment diet and freely drank water .The rats in Mg group were fed with a standard diet and drank magnesium citrate solution .The Con group was fed with a standard diet and normal water.The general conditions and the characteristics of stool were observed every day .The grip strength and motor activity of the rats were measured after diarrhea for 14 d.The trans-epithelial resistance of the colonic mucosa was detected by Us-sing chamber .The pathological changes of villus height and villus surface area in the intestine were observed with hemato -xylin-eosin staining .RESULTS:The rats in both diarrhea groups displayed the clinical manifestations of slow increase in body weight , spare and dull hair , lacking in strength and physical inactivity .The grip strength and motor activity of the rats in both diarrhea groups decreased significantly compared with Con group .The diarrhea rate and diarrhea index of the rats in the 2 diarrhea groups were both significantly higher than those in Con group (P<0.05).Moreover, the diarrhea index in Lac group was significantly increased compared with Mg group .The thickness of intestinal mucosa in Lac group was thinner than that in Mg group at the same time .However , the damage of intestinal villi in Mg group was more serious than that in Lac group.CONCLUSION:Both methods of lactose enrichment-induced diarrhea and magnesium citrate-induced diarrhea meet the requirement of chronic osmotic diarrhea , but there are different advantages between the diarrhea models .There-fore, we should select the correct diarrhea model to meet different aims of the experiments .关键词
慢性腹泻/乳糖/柠檬酸镁Key words
Chronic osmotic diarrhea/Lactose/Magnesium citrate分类
医药卫生引用本文复制引用
刘舒,高媛,肖露,杨亭,李廷玉,陈洁..两种慢性渗透性腹泻大鼠模型建立方法的比较[J].中国病理生理杂志,2016,32(8):1484-1491,8.基金项目
国家自然科学基金资助项目（No．81470799）；重庆市渝中区科委项目 （）
