中国动物检疫2016，Vol.33Issue(8)：72-77,6.DOI:10.3969/j.issn.1005-944X.2016.08.021
重组酶聚合酶扩增技术在疾病快速检测中的研究进展
Research Progress of Recombinant Enzyme Polymerase Ampliifcation(RPA) in Rapid Detection of Diseases
摘要
Abstract
Recombinase polymerase amplification(RPA) is a nucleic acid amplification technique developed in recent years. The test retains the highest levels of sensitivity and specificity,while adding extraordinary speed and portability. The assay generally amplified target DNA at room temperature in 10~20 minutes. It reduces the need of infrastructure and resources and is particularly suitable for rapid diagnosis in veterinary,food safety,biological defense,agriculture and otherfields. The principle of RPA as well as the current application in the detection of viruses,bacteria,parasites and other pathogens were introduced,and the future use of testing in point of care andfield settings were prospected.关键词
重组酶聚合酶扩增/快速诊断/应用Key words
recombinase polymerase amplification/rapid diagnosis/application分类
医药卫生引用本文复制引用
樊晓旭,赵永刚,李林,吴晓东,王志亮..重组酶聚合酶扩增技术在疾病快速检测中的研究进展[J].中国动物检疫,2016,33(8):72-77,6.基金项目
潜在入侵的畜禽疫病口岸监测技术研究 （）
