中国动物检疫2016，Vol.33Issue(8)：82-85,4.DOI:10.3969/j.issn.1005-944X.2016.08.023
鸡传染性支气管炎病毒广西株N基因的克隆与序列分析
Cloning and Sequence Analysis on N Gene of an Infectious Bronchitis Virus Guangxi Isolate
摘要
Abstract
One pair of primers for amplifying the N gene of IBV based on the sequence in GenBank was designed. The cDNA fragments of N gene of IBV strain isolated from Guangxi province were amplified by RT-PCR,then the amplified fragments were cloned into pMDl8-T vector and the recombinant plasmids were sequenced. The results showed that the N gene from all of the IBV isolates consisted of 1230 bp,coding for 409 amino acid. Compared with that of the other published IBV N genes,the homology of nucleotide and amino acid sequence of the isolate were 83.8%~99.9% and 88.4%~99.6% respectively. The isolate strain was closely related to LX4 and BJ and had relatively distant phylogentic relationship with the immune strain which was grouped in other clade. These results suggested that the isolate was a new variant of IBV.关键词
鸡传染性支气管炎病毒/N基因/基因克隆/序列分析Key words
avian infectious bronchitis virus/N gene/genetic cloning/sequence analysis分类
农业科技引用本文复制引用
屈素洁,邹联斌,胡杰,粟艳琼,尹彦文,陆文俊,李军,施开创,莫胜兰..鸡传染性支气管炎病毒广西株N基因的克隆与序列分析[J].中国动物检疫,2016,33(8):82-85,4.基金项目
广西区水产畜牧兽医局科技项目 （）
