中国动物检疫2016，Vol.33Issue(8)：90-93,4.DOI:10.3969/j.issn.1005-944X.2016.08.025
两种不同刺激剂对鸡淋巴细胞分泌细胞因子的影响
Effect of Two Kinds of Stimuli on Cytokines Secreted by Chicken Peripheral Blood Lymphocytes
摘要
Abstract
Objective]To compare the effect of two kinds of stimuli,concanavalin A(Con A)and phytohemaggluti-nin(PHA)on cytokine secreted by chicken lymphocyte. [Methods] First,peripheral blood lymphocytes from chick-ens were separated by lymphocyte separation liquid and cultured with Con A and PHA for 48 h,respectively,then the cell RNAs were extracted and the reverse transcription was carry out. Secondly,a real time PCR was performed for detection of the RNA transcription level in the chicken lymphocytes stimulated by Con A and PHA. Finally,the effect of two kinds of stimuli on cytokines secretion was compared with molecular biology software. [Results]Chicken lym-phocytes with higher purity and a survival rate were obtained with the used method. The transcription levels of tested cy-tokines stimulated by Con A were evidently higher than that by PHA(P < 0.01). Moreover,PHA could not increase cytokine transcription levels(P>0.05)except of IL-4(P < 0.01). [Conclusion]Con A can enhance the transcription levels of cytokine genes and is a be better stimulus for chicken lymphocytes than PHA. All these results provide methods for the further study of chic ken lymphocytes in vitro.关键词
鸡T细胞/细胞因子/荧光定量PCRKey words
chicken T cell/cytokine/fluorescence quantitative PCR分类
农业科技引用本文复制引用
刘洪明,王官晓,王俊卿,杨玉华,郭善娜,徐晓静..两种不同刺激剂对鸡淋巴细胞分泌细胞因子的影响[J].中国动物检疫,2016,33(8):90-93,4.基金项目
国家自然科学基金青年项目（31201888）；国家自然科学基金面上项目 （）
