中国动物检疫2016，Vol.33Issue(8)：94-97,4.DOI:10.3969/j.issn.1005-944X.2016.08.026
猪细小病毒PCR检测方法的建立与应用
Establishment and Application of PCR Methods for Porcine Parvovirus Detection
摘要
Abstract
Based on the VP2 genomic sequence of porcine parvovirus that published in GenBank, a pair of primers were designed by the Primer5.0 software,and the target fragment of amplification was 591 bp. The PPV PCR detective method was established by specificity, sensibility,reproducibility and parallelism assay. The conformity test results showed that the PCR method was better than HA,VI and IHA. Using the method to test 215 clinical samples,there were 25 positive samples,with a detection rate of 11.63%. All Results showed that this method could be used in the diagnosis and epidemiological survey of PPV.关键词
猪细小病毒/VP2基因/聚合酶链式反应/检测Key words
porcine parvovirus/VP2 gene/PCR/detection分类
农业科技引用本文复制引用
邹敏,李陆梅,宫晓,王红,杨旭兵,范根成..猪细小病毒PCR检测方法的建立与应用[J].中国动物检疫,2016,33(8):94-97,4.基金项目
青岛市民生计划课题--猪细小病毒灭活疫苗的研制及产业化 （）
