中国畜牧兽医2016，Vol.43Issue(8)：1929-1937,9.DOI:10.16431/j.cnki.1671-7236.2016.08.001
水牛 STA T4基因克隆分析及其真核表达载体的构建
Cloning and Construction of Eukaryotic Expression Vector of Buffalo STAT4 Gene Cloning and Construction of Eukaryotic Expression Vector of Buffalo STAT4 Gene
摘要
Abstract
In order to elucidate the function and molecular mechanisms of buffalo signal transducer and activator of transcription 4 (STAT4) gene during folliculogenesis ,embryogenesis and lacto‐genesis ,buffalo STA T4 gene was studied with 3′‐RACE ,bio‐informatics analysis ,eukaryotic vec‐tor construction and cell transfection technologyin this study .The results showed that the coding region of buffalo STAT4 was 2 247 bp ,3′‐was 268 bp ,and encoded 748 amino acids ;BLAST anal‐ysis showed that the buffalo STA T4 gene shared 99% ,99% ,99% ,95% ,93% ,93% and 92% of similar nucleotide sequence with that of Bos taurus ,Capra hircus ,Ovis aries ,Sus scrofa ,Equus caballus ,Canis lupus and Homo sapiens ,respectively .STAT4 protein was weakly acidic ,without signal peptide ,located in the cytoplasmic ,and with the presence of STAT_int ,STAT_alpha , STAT_bind and SH2_STAT4 domain .bta‐miR‐200a ,bta‐miR‐2429 and bta‐miR‐2410 and so on were potential microRNAs of STAT4 3′‐UTR .The buffalo STAT4 eukaryotic expression vector was successfully constructed ,after transfected into HEK293T cell lines ,STAT4‐EGFP fusion protein was detectable .关键词
水牛/STA T4基因/克隆/生物信息学分析/载体构建Key words
buffalo/STAT4 gene/cloning/bio-informatics analysis/vector construction分类
生物科学引用本文复制引用
朱鹏,庞春英,段安琴,邓廷贤,陆杏蓉,梁贤威..水牛 STA T4基因克隆分析及其真核表达载体的构建[J].中国畜牧兽医,2016,43(8):1929-1937,9.基金项目
农业部转基因重点项目（2014ZX0801012B）；广西国合桂科合（14123001-5）；水牛人才 （）
