中国畜牧兽医2016，Vol.43Issue(8)：1944-1950,7.DOI:10.16431/j.cnki.1671-7236.2016.08.003
两种布鲁氏菌弱毒株侵染小鼠巨噬细胞过程的荧光表征及分析
The Fluorescent Characterization and Analysis of Two Brucella Attenuated Vaccine Infecting Mouse Macrophagocyte
摘要
Abstract
The experiment was conducted to discuss the difference of binding time of green fluo‐rescent protein B.melitensis M5 (GFP‐M5) and B.abortus S19 (GFP‐S19) infecting the mouse macrophagocyte (RAW264.7) ,lysosome ,endoplasmic reticulum and golgi body in the initial stage and compare the binding rate of GFP‐M5 ,GFP‐S19 with organelle in different timeline ,respec‐tively ,by confocal laser scanning microscope (CLSM) and flow cytometry.The result showed that GFP‐M5 and GFP‐S19 were successfully constructed.The intracellular survival ability of Brucella M5 , Brucella S19 , GFP‐M5 and GFP‐S19 were not obvisouly affected after infecting RAW264.7.GFP‐M5 and GFP‐S19 could enter the macrophagocyte in 30 mins ,and in 2 h the Brucella could reach lysosome ,endoplasmic reticulum and golgi body.In addition ,the binding time for two attenuated vaccine did not show differences in 1 ,2 ,3 and 4 h.The content of GFP+cell produced by RAW264.7 infected by GFP‐M5 and GFP‐S19 did not show significant differ‐ences (P>0.05).Therefore ,the two strains did not have significant differences in the invasion ability in the initial stage of infecting host cell .关键词
布鲁氏菌/绿色荧光蛋白(GFP)/RAW264.7/共聚焦显微镜/流式细胞仪Key words
Brucella/green fluorescent protein/RAW264.7/confocal laser scanning microscope/flow cytometry分类
农业科技引用本文复制引用
胡梦薇,刘朋涛,严国,高剑峰..两种布鲁氏菌弱毒株侵染小鼠巨噬细胞过程的荧光表征及分析[J].中国畜牧兽医,2016,43(8):1944-1950,7.基金项目
国家重点基础研究发展计划（973计划） （）
