中国畜牧兽医2016，Vol.43Issue(8)：1960-1966,7.DOI:10.16431/j.cnki.1671-7236.2016.08.005
小鼠 Oc t-1基因 CDS 区克隆与生物信息学分析
Cloning and Bioinformatics Analysis on CDS of Oct-1 Gene in Mouse
摘要
Abstract
In order to explore the biological characteristics of Oct‐1 gene in mouse ,CDS region of Oct‐1 gene in mouse was cloned .And the sequence ,physicochemical property ,subcellular localiza‐tion ,target genes and conservative structure domain of Oct‐1 gene were analyzed by bioinformatics method to predict w hether the Oct‐1 gene could regulate the production of melanin .T he results showed that the length of Oct‐1 gene in mouse was 2 313 bp .The formula of Oct‐1 protein was C3425 H5606 N976 O1163 S15 ,which was an unstable and soluble protein .The secondary structure of Oct‐1 mainly contained random coil and α‐helices .Subcellular localization of Oct‐1 was mainly in the nucleus ,w hich Oct‐1 might play a role of signal transducer or transcription regulation in the ener‐gy metabolism and cofactor biosynthesis .The Oct‐1 gene regulated the expression of 13 genes associated with the melanin formation of mouse .The protein was encoded by Oct‐1 gene including a homeo‐domain and a POU domain .There were binding sites of Oct‐1 on the promoters of MITF ,TYR ,TYRP1 and TYRP2 .Oct‐1 might interact with Brf2 ,Pou2af1 and Tbp to regulate the colour gene expression .We inferred that the transcription factor Oct‐1 played an important role in melanogenesis and these results provided a theoretical basis for studying the coat colour formation mechanism .关键词
Oct-1基因/黑色素细胞/基因克隆/生物信息学Key words
Oct-1 gene/melanocytes/gene cloning/bioinformatics分类
生物科学引用本文复制引用
杨玉静,聂瑞强,谢建山,范瑞文,董常生..小鼠 Oc t-1基因 CDS 区克隆与生物信息学分析[J].中国畜牧兽医,2016,43(8):1960-1966,7.基金项目
国家高科技研究发展计划（2013AA102506）；公益性行业（农业）科研专项（201303119）；山西农业大学创新团队 （）
