中国畜牧兽医2016，Vol.43Issue(8)：1975-1982,8.DOI:10.16431/j.cnki.1671-7236.2016.08.007
水牛 Keap1基因的克隆、序列分析及组织表达研究
Cloning and Sequence Analysis of Buffalo Keap1 Gene and Investigation of Its Expression Pattern in Different Tissues
摘要
Abstract
In this study ,the CDS sequence of buffalo Keap1 gene was cloned and analyzed ,then its expression pattern in different tissues was also investigated.A pair of primers of buffalo Keap1 gene was designed based on the nucleotide sequence of Bos taurus Keap1 gene from GenBank ,and then the buffalo Keap1 gene was amplified.Using the bioinformation techniques ,the gene se‐quence and the protein structure were analyzed.The expression level of Keap1 gene in different tissues were detected with Real‐time quantitative PCR.The results showed that the length of buf‐falo Keap1 gene coding sequence was 1 875 bp and encoded 624 amino acids.The multiple se‐quence alignment results showed that buffalo Keap1 gene shared 99% ,96% ,92% and 90% of similar nucleotide sequence with that of Bos taurus ,Ovis aries ,Sus scrofa and Homo sapiens ,re‐spectively.And the phyogenetic tree also showed the conservatism between several different spe‐cies.The second structure of buffalo Keap1 protein was predicted as 24 alpha regions ,40 beta re‐gions ,38 turn regions and 27 coil regions.In addition ,the results of Real‐time quantitative PCR showed that Keap1 mRNA exists in all seven tissues ,but the most abundant expression was in heart and the minimal expression was in liver and spleen.The results provided an foundation for further study of Keap1‐Nrf2‐ARE signal pathway ,for enhancing the ability of antioxidant of buf‐falo embryo in v itro culture.关键词
水牛/Keap1基因/克隆/分析/表达Key words
buffalo/K eap1 gene/clone/analysis/expression分类
生物科学引用本文复制引用
赵鑫,阮子芸,郭振伟,周文婷,秦希玲,牛向丽,陆凤花,石德顺..水牛 Keap1基因的克隆、序列分析及组织表达研究[J].中国畜牧兽医,2016,43(8):1975-1982,8.基金项目
国家高技术研究发展计划“863”项目（2011AA100607）；国家自然科学基金项目（31560633）；广西科学基金（2012GXNSFFA060004）；广西研究生教育创新计划项目（YCBZ2015004）；广西计生委科研项目 （）
