中国畜牧兽医2016，Vol.43Issue(8)：2032-2037,6.DOI:10.16431/j.cnki.1671-7236.2016.08.015
新生牛血中 Cu／Zn-SOD 的提纯及肝素、壳聚糖的逐层自组装包裹
Separation and Purification of Cu/Zn-SOD from Newborn Bovine Blood and Prepatation of HEP and CHI Using Layer-by-layer Self-assembly Technique
摘要
Abstract
In order to improve the stability of super oxide dismutase (SOD) with some modifica‐tion ,we used thermal denaturation extraction method to extract Cu/Zn‐SOD from newborn bovine blood ,and used layer‐by‐layer self‐assembly technique ,heparin and chitosan as material to modifi‐cate the SOD which separated and purified from newborn bovine blood .The results showed that compared with the natural SOD ,the modified SOD increased 3 .3 times in the thermal stability ;In acid and alkaline ,under the condition of pH 3 .0 and pH 11 .0 ,the acid‐base resistance property of the modified SOD raised 2 .0 times;On the antitrypsin hydrolysis ability ,the relative enzyme ac‐tivity of modified SOD had increased 2 .7 times than natural SOD .The modified SOD with shell outside made of heparin and chitosan by physical modification could get better effect ,and had the strong mechanical strength ,better biocompatible ,outer surface of chitosan was helpful to cross the cell mem‐brane transport ,and it could be applied to the development of oral dosage forms of SOD .关键词
SOD/肝素/壳聚糖/逐层自组装技术/相对酶活性Key words
SOD/heparin/chitosan/layer-by-layer self-assembly technique/relative enzyme activity分类
农业科技引用本文复制引用
侯静,林春丽,白志军,魏建民,张永明,张峰..新生牛血中 Cu／Zn-SOD 的提纯及肝素、壳聚糖的逐层自组装包裹[J].中国畜牧兽医,2016,43(8):2032-2037,6.基金项目
国家自然科学基金（21171086、81160213）；内蒙古自治区草原英才工程项目 （）
