中国畜牧兽医2016，Vol.43Issue(8)：2038-2046,9.DOI:10.16431/j.cnki.1671-7236.2016.08.016
响应面法优化里氏木霉固体发酵产α-半乳糖苷酶培养条件
Optimization of Culture Conditionsin in Trichoderma reesei Solid-state Fermentation Producing α-galactosidase using Response Surface Method
摘要
Abstract
The culture conditions of Trichoderma reesei solid‐state fermentation to produce α‐gal‐actosidase were optimized by response surface method .First of all ,according to the single fac‐tors ,the experiments were designed as Plackett ‐ Burman and three main factorswere selected out :The ratio of corn cob and bagasse carbon source ,the ratio of beef extract and ammonium ni‐trogen source and the fermentation initial pH .On the basis of using the method of the steepest uphill path approximation maximum response area ,the response surface method was used to de‐termine the main factors of the interaction between the main factors and the optimal culture condi‐tions .The results showed that the optimized culture conditions in Trichoderma reesei solid‐state fermentation producing α‐galactosidase were :the ratio of carbon source (namely corn cobsand to bagasse ratio) was 3 .86 ,the ratio of nitrogen source (beef paste to ammonium sulfate) was 1 .17 ,and the initial pH was 9 .12 .After optimization ,the α‐galactosidase activity produced in Trichoderma reesei solid‐state fermentation could be up to 342 .98 U/g and were close to 353 .43 U/g , the model prediction .It was increased by 113 .91% than before optimization .In conclusion ,the most important factors in Trichoderma reesei solid‐state fermentation were carbon source ,nitro‐gen source and pH value ,which interacted each other .The activity of α‐galactosidase was maxi‐mum when the ratio of carbon source was up to 3 .86 ,the ratio of nitrogen source was up to 1 .17 and pH value reach to 9 .12 .关键词
响应面/α-半乳糖苷酶/固体发酵/里氏木霉Key words
response surface/α-galactosidase/solid-state fermentation/T richoderma reesei分类
生物科学引用本文复制引用
莫海飞,陈炼红,黄林,伍红..响应面法优化里氏木霉固体发酵产α-半乳糖苷酶培养条件[J].中国畜牧兽医,2016,43(8):2038-2046,9.基金项目
西南民族大学自然科学基金项目里氏木霉纤维素酶基因表达调控关键因子研究 （）
