中国畜牧兽医2016，Vol.43Issue(8)：2053-2059,7.DOI:10.16431/j.cnki.1671-7236.2016.08.018
转双基因（pGH／IGF-Ⅰ）猪胎儿成纤维细胞系的建立及鉴定
Establishment and Identification of a Fetal Fibroblast Cell Line with Double Transgenic (pGH/IGF-Ⅰ) Porcine
摘要
Abstract
This study was aimed to establish a fetal fibroblast cell line of double transgenic (pGH/IGF‐Ⅰ) pigs ,and reserve fibroblast cells of double transgenic pigs for the follow‐up study.A method of trypsin digestion was adopted to isolate and culture body tissues from pregnant por‐cine ,and porcine fetal fibroblasts were isolated successfully through primary culture ,subculturing and cryopreservation.T he morphological observation ,determination of viability before cryopreser‐vation and after recovery ,dynamic grow th analysis ,vimentin immunohistochemistry and microbial contamination detection were all done to study the biological characteristics of the cell line.The results showed that the fibroblasts were cultured and isolated successfully by trypsin digestion and differential centrifugation.The cell viability before cryopreservation and after recovery were 94.3% and 91.2% ,respectively.The growth curve was sigmoidal ,and experienced the incubation period ,exponential growth period and platform three stages.The vimentin immunohistochemistry was positive ,the microbial contamination detection were all negative.The results indicated that a fibroblast cell line of double transgenic porcine was successfully established.关键词
转双基因猪/成纤维细胞系/细胞培养/生物学特性Key words
double transgenic porcine/fibroblast cell line/cell culture/biological characteristics分类
生物科学引用本文复制引用
李晓玲,于光辉,孙金海..转双基因（pGH／IGF-Ⅰ）猪胎儿成纤维细胞系的建立及鉴定[J].中国畜牧兽医,2016,43(8):2053-2059,7.基金项目
国家转基因生物新品种培育科技重大专项（2014ZX08006-003）；山东省现代农业产业技术体系生猪创新团队建设项目 （）
