中国畜牧兽医2016，Vol.43Issue(8)：2104-2111,8.DOI:10.16431/j.cnki.1671-7236.2016.08.024
绵羊卵母细胞超低温冷冻对四跨膜蛋白 CD 9表达的影响
Effect of Cryopreservation on the Expression of Tetraspanin CD 9 in Sheep Oocytes
摘要
Abstract
The experiment was aimed to explore the expression of CD9 in the follicles at different developmental stages ,and investigate the effect of in v itro maturation and cryopreservation on CD9 .The expression of CD9 protein in the follicle was detected by immunohistochemistry tech‐nique ,the expression of CD9 mRNA was detected by Real‐time quantitative PCR ,CD9 protein was detected by Western blotting .The results showed that the CD9 fluorescence signal was de‐tected by immunohistochemistry technique ,and the fluorescence signal gradually increased with the maturation of the follicle ,and fluorescence signal was the strongest in mature follicle ;Real‐time quantitative PCR showed that the expression of CD9 mRNA in fresh M Ⅱ oocytes was signif‐icantly increase (P<0 .05) ,and was the least in frozen GV oocytes ;The result of Western blot‐ting indicated that CD9 protein was the most in fresh M Ⅱ oocytes and the expression of frozen GV oocytes was the least ,consistent with the results of Real‐time quantitative PCR .Cryopreser‐vation of oocytes was more extensive than embryo cryopreservation .Tetraspanins CD9 played a very important role in sperm egg fusion .The above experimental results showed that the cryopr‐eservation made damage to oocytes ,decrease content of tetraspanins CD9 protein ,and CD9 injury was one of the important reasons for the decline of fertilization rate .关键词
CD9/卵巢/卵母细胞/体外成熟/免疫组化/Western blotting/实时荧光定量PCRKey words
CD9/ovary/oocytes/in vitro maturation/immunohistochemistry/Western blotting/Real-time quantitative PCR分类
农业科技引用本文复制引用
乌丽雅苏,乌亚罕,史振丹,爱伦高娃,扎木嘎,乌云斯钦,恩和玛日特,娜仁花..绵羊卵母细胞超低温冷冻对四跨膜蛋白 CD 9表达的影响[J].中国畜牧兽医,2016,43(8):2104-2111,8.基金项目
国家自然基金项目 （）
