中国畜牧兽医2016，Vol.43Issue(8)：2143-2149,7.DOI:10.16431/j.cnki.1671-7236.2016.08.029
宫净灌注剂急性毒性和长期毒性试验研究
Experimental Study on Acuet Toxicity and Long-term Toxicity of Gongjing Perfusion
摘要
Abstract
The purpose of this paper was to study the acute toxicity in mice and long‐term toxicity in rats of Gongjing perfusion to evaluate the clinical safety of medication and provide theoretical basis for clinical application .In the acute toxicity experiment ,mice were given once the approxi‐mate lethal dose of Gongjing perfusion to measure the acute toxicity and the maximal tolerance dose (MTD) .In the long‐term toxicity observation ,80 rats were divided into four groups :low‐dose ,middle‐dose ,high‐dose test groups of Gongjing perfusion (2 .5 ,5 .0 ,10 .0 g/(kg · d) ) and control group with distilled water intragastric administration for 30 days .During the experimental period ,the appearance ,behavior and feces condition of rats were observed and recorded .Each rat was weighted every week ,and the average daily gain were calculated .After 30 days ,rats were exe‐cuted and taken blood in their hearts ,hematology and blood chemistry were detected .The results showed that the mice were all survived and LD50 was not measured ,the M TD was 40 g/kg .In the long‐term toxicity test ,compared with the control group ,there were no significant difference in various index except individual index and the pathological examination revealed that no obvious pathological changes related to drug toxicity .So Gongjing perfusion had not long‐term toxicity and acute toxicity under this experiment condition ,w hich suggested Gongjing perfusion had a good clinical safety .关键词
宫净灌注剂/急性毒性/长期毒性/安全性Key words
Gongjing perfusion/acute toxicity/long-term toxicity/safety引用本文复制引用
吕安§,张志聪§,宫平,张彤,刘艺林,姜晓茜,李德琰,刘凤华..宫净灌注剂急性毒性和长期毒性试验研究[J].中国畜牧兽医,2016,43(8):2143-2149,7.基金项目
国家“十二五”科技支撑计划重点项目 （）
