中国畜牧兽医2016，Vol.43Issue(8)：2156-2163,8.DOI:10.16431/j.cnki.1671-7236.2016.08.031
INH 表位多肽疫苗抗体间接 ELISA 测定方法的建立及优化
Establishment and Optimization of INH Epitope Peptide Vaccine for Detection of Antibody
摘要
Abstract
In this study ,an indirect ELISA method was established to detect inhibin hormone (INH) epitope peptide vaccine antibody ,it would provide oretical reference for the determination of Fine‐wool sheep after active immune body INH epitope peptide vaccine antibody .On the basis of the predecessors ,using indirect ELISA method to determinate serum IN H epitope peptide an‐tibody levels of sheep ,and through control different experimental conditions to look for the best experimental conditions .Through explorating the experimental conditions ,finally ,the testing experiment conditions were determined ,which was blocked solution with skimmed milk powder , IN H and GnIH synthetic peptides dilution degrees for 20 000 times ,the optimum reaction time was 60 min ,the best color action time was 15 min .In this experiment ,a kind of method to detec‐tion antibody in the body after INH active immune sheep was built ,it would provide a reference for future research .关键词
抑制素/细毛羊/主动免疫/生殖激素Key words
inhibin hormone/Fine-wool sheep/active immunization/reproductive hormone分类
农业科技引用本文复制引用
张玲玲,韩吉龙,刘善博,杨博辉,岳耀敬,冯瑞林,李红峰,郭婷婷,袁超,牛春娥,刘建斌,孙晓萍..INH 表位多肽疫苗抗体间接 ELISA 测定方法的建立及优化[J].中国畜牧兽医,2016,43(8):2156-2163,8.基金项目
甘肃省青年自然基金“非繁殖季节 GnI H 基因免疫对藏羊卵泡发育的调节机制”；国家绒毛用羊产业技术体系分子育种岗位（CARS-40-03）；中国农业科学院“细毛羊资源与育种”创新工程 （）
