中国畜牧兽医2016，Vol.43Issue(8)：2164-2169,6.DOI:10.16431/j.cnki.1671-7236.2016.08.032
酵母诱导表达鸡 IFN-α抗 IBDV 效果及其对信号分子 PI3K 和 NF-κB 的影响
Study on the Anti-IBDV Activity of Chicken IFN-α and Its Effects on Signaling Molecule PI3K and NF-κB
摘要
Abstract
To investigate the anti‐IBDV activity of IFN‐α and its effects on chicken lymphocyte signaling molecule PI3K and NF‐κB activity ,forty 10‐day‐old non‐immune chickens were random‐ly separated into two groups :Blank control group and IFN‐α group .The chicken of two groups were inoculated with IFN‐αand saline via intramscular injection for three days ,respectively .Then they were inoculated with IBDV .From 13 to 18‐day‐old ,the lymphocytes were isolated from pe‐ripheral blood of chicken .The lymphocyte proliferation of chicken were detected by the MTT method ,the PI3K and NF‐κB p65 concentration in lymphocyte and the IBDV‐Ag of serum were detected at different time with ELISA .The results showed that compared with the blank control group ,IFN‐αcould stimulate chicken lymphocyte proliferation and the PI3K and NF‐κB p65 con‐centration in chicken lymphocyte were extremely significantly increased before infection ( P<0 .01) .The lymphocyte proliferation and the expression of PI3K and NF‐κB p65 in cell after IBDV infection were extremely significantly higher than before (P<0 .01) .IFN‐α could extremely sig‐nificantly inhabit the proliferation of IBDV‐Ag ( P< 0 .01 ) .IFN‐α could significantly promote lymphocyte transformation by increasing PI3K concentration and the biological activity of lym‐phocyte transformation associated with the PI3K and NF‐κB p65 expression ,activation and nu‐cleo‐cytoplasmic transport .It also could reduce IBDV‐Ag proliferation in chickens .The study built foundation for IFN‐αprotein immuno enhancement and anti‐viral activity .关键词
IFN-α/PI3K/核转录因子/NF-κB p65/传染性法氏囊病Key words
IFN-α/PI3K/nuclear factor/NF-κB p65/IBD分类
农业科技引用本文复制引用
韩海锋,李银聚,程相朝,张春杰,余祖华,杨惠,杨荣恩,付威..酵母诱导表达鸡 IFN-α抗 IBDV 效果及其对信号分子 PI3K 和 NF-κB 的影响[J].中国畜牧兽医,2016,43(8):2164-2169,6.基金项目
河南省科技攻关项目 （）
