中国畜牧兽医2016，Vol.43Issue(8)：2190-2195,6.DOI:10.16431/j.cnki.1671-7236.2016.08.036
蜂胶黄酮对宿主细胞抗病毒能力的影响
Effects of Propolis Flavone on Antiviral Ability of Host Cell
摘要
Abstract
This experiment was conducted to study the effects of propolis flavone (PF) on antivi‐ral ability of host cell .First ,the maximal safe concentration of PF to CEF and PK‐15 was deter‐mined by MTT method .Then PF was diluted to five concentrations (from 250 to 15 .6 μg/mL) with maintenance medium and acceded to foster system of CEF or PK‐15 with NDV ,IBDV , TGEV and PPV ,respectively ,and the cell viability was observed by MTT method at 24 ,48 and 72 h after infection as the index of high antiviral ability of host cell .The results showed that PF could promote the antiviral ability of host cell .And the effects were related to PF concentration , as the concentration increased ,the effects were better .The antiviral activity of PF to NDV and TGEV with envelope in host cell existed in infectious earlier stage ,while for IBDV and PPV with‐out envelope ,the activity appeared in infectious later stage .T he results indicated that the antiviral effect period and mechanism of propolis flavone was different for different viruses ,propolis fla‐vone could be use for the prevention of NDV and TGEV ,and for the treatment of IBDV and PPV .关键词
蜂胶黄酮/宿主细胞/抗病毒Key words
propolis flavone/host cell/antiviral分类
农业科技引用本文复制引用
郭振环,马霞,王恬,沈志强..蜂胶黄酮对宿主细胞抗病毒能力的影响[J].中国畜牧兽医,2016,43(8):2190-2195,6.基金项目
山东省滨州畜牧兽医研究院博士后科研启动基金 （）
