中国循证儿科杂志2016，Vol.11Issue(4)：290-294,5.DOI:10.3969/j.issn.1673-5501.2016.04.010
自然杀伤细胞与丙型肝炎病毒感染的Huh7．5细胞体外共培养后的功能变化
Functional changes in natural killer cells co-cultured with Huh7 . 5 cells infected with the hepatitis C virus in vitro
摘要
Abstract
Objective The numbers,subsets and function of natural killer( NK)cells altered in chronic hepatitis C virus ( HCV)infected patients which indicating its association with chronic HCV infection,but the exact mechanisms remain unclear. To explore the functional effect of HCV infection on NK cells in vitro and the possible mechanism behind it. Methods Human hepatoma Huh7. 5 cells were transfected with genomic RNA from cell culture adapted JC1 genotype 2a HCV strain( Huh7. 5-HCVcc)and co-cultured with NK cells isolated from whole peripheral blood of concentrated healthy donors. The functional effect of HCV infection on NK cells was assessed by testing cytokine-secreting and cytotoxicity before and after co-culture. Results When co-cultrued with Huh7. 5-HCV of MOI 4. 8,the levels of IFN-γ,TNF-α and IL-10 secreted by NK cells were inhibited. The inhibition of secreting IFN-γwas specially significant at 6(P<0. 001),9(P<0. 001)and 12(P=0. 001)hours co-cultured with Huh7.5-HCV. The inhibition of secreting TNF-α was specially significant 6 h(P <0.001),9 h(P <0.001)and 12 h(P =0. 001)after co-cultured with Huh7. 5-HCVcc. And the significant inhibition of secreting IL-10 appeared at 6 h(P<0. 001)and 9 h(P=0. 006)after co-cultured with Huh7. 5-HCVcc. The strongest cytokine-secreting inhibition appeared 6 h after co-cultured with Huh7. 5-HCVcc,the inhibition rate was 24. 1%,20. 7% and 24. 3%,respectively. The cytotoxicity decreased significantly 6 h after co-cultured with Huh7. 5-HCVcc(P=0. 023),and the average inhibition rate was 16. 6%. Conclusion When in vitro cocultrued with Huh7. 5-HCVcc of MOI 4. 8,the NK cells functions of IFN-γ,TNF-αand IL-10 cytokine-secreting and cytotoxicity were inhibited. The degree of inhibition was different at different time points,which indicated that NK cells may act differently during HCV infection.关键词
自然杀伤细胞/丙型肝炎病毒/Huh7. 5细胞/共培养Key words
Natural killer cells/Hepatitis C virus/Huh7. 5 cells/Co-culture引用本文复制引用
胡姚,张婷,叶颖子,王晓红,俞蕙..自然杀伤细胞与丙型肝炎病毒感染的Huh7．5细胞体外共培养后的功能变化[J].中国循证儿科杂志,2016,11(4):290-294,5.基金项目
上海市科委自然科学基金12ZR1403500 （）
