中国中医药信息杂志2016，Vol.23Issue(9)：61-64,4.DOI:10.3969/j.issn.1005-5304.2016.09.015
BALB/c小鼠阴虚型过敏性哮喘模型的建立与评价
Establishment and Evaluation of BALB/c Mouse Model of Anaphylactic Asthma with ;Yin-deficiency Syndrome
摘要
Abstract
Objective To establish and evaluate a BALB/c mouse model of anaphylactic asthma with yin-deficiency syndrome. Methods Ovalbumin (OVA) was injected to sensitize and was inhaled to stimulate to replicate the BALB/c mouse model of anaphylactic asthma. Thyroxin was used for gavage during late stimulation to replicate the BALB/c mouse model of asthma with yin-deficiency syndrome. On the basis of monitoring food and water intake and body weight, asthma related indexes, such as asthmatic behaviors, respiratory function, lung histopathology, were detected, and yin-deficiency syndrome related indexes, such as cAMP and cGMP, and pulmonary fluid clearance were examined. Results The BALB/c mouse model of asthma with yin-deficiency syndrome showed obvious asthma symptoms; comprehensive scores of asthmatic behaviors increased significantly;inhaling peak flow, exsufflation peak flow, and tidal volume decreased significantly; lung tissue histopathology showed obvious inflammatory response. Meanwhile, food and water intake of BALB/c mouse model of asthma with yin-deficiency syndrome increased significantly;body weight increased slowly;wet/dry ratio of lung tissue decreased;cAMP and cAMP/cGMP increased, while cGMP decreased, which showed that the mice were in the condition of yin-deficiency and yang-excess. Conclusion Combining thyroxin at the basis of sensitivity induced by OVA is a good pattern to successfully replicate the BALB/c mouse model of anaphylactic asthma with yin-deficiency syndrome.关键词
阴虚型过敏性哮喘/动物模型/BALB/c小鼠Key words
anaphylactic asthma with yin-deficiency syndrome/animal model/BALB/c mice分类
医药卫生引用本文复制引用
王志旺,妥海燕,任远,李荣科,刘雪枫,程小丽..BALB/c小鼠阴虚型过敏性哮喘模型的建立与评价[J].中国中医药信息杂志,2016,23(9):61-64,4.基金项目
国家自然科学基金（81460668）；甘肃省自然科学基金 （）
