中国中医药信息杂志2016，Vol.23Issue(9)：74-77,4.DOI:10.3969/j.issn.1005-5304.2016.09.018
扶正化瘀胶囊对心肌梗死大鼠Rock1、Rock2表达的影响
Effects of Fuzheng Huayu Capsule on Expressions of Rock1 and Rock2 in Rats with Myocardial ;Infarction
摘要
Abstract
Objective To observe the effects of Fuzheng Huayu Capsule on the expressions of the main roles, Rock1 and Rock2, in RhoA/Rock signal transduction pathway in rats with myocardial infarction (MI);To explore the possible mechanism of Fuzheng Huayu Capsule to improve ventricular remodeling in myocardial fibrosis. Methods The MI model was induced by ligation of the left coronary artery. Rats with MI were randomly divided into the model group, Fuzheng Huayu group and Fasudil hydrochloride group. A sham-operation group threading without ligation was setting as a control group, with eight rats in each group. The rats were treated with corresponding medicine for 4 weeks from the second day after modeling. The expression of Rock1, Rock2 and its mRNA were detected by immunohistochemical and real-time PCR method. Results The protein expression of Rock1 and Rock2 in model group were significantly higher than those in the sham-operation group (P<0.05). The protein expression of Rock1 and Rock2 in the Fuzheng Huayu group and Fasudil hydrochloride group were lower than those in the model group. The mRNA expression of Rock2 was significantly higher in the model group than that in the sham-operation group (P<0.05). The mRNA expression of Rock1 in the Fasudil hydrochloride group was lower than that in the model group (P<0.05). The mRNA expression of Rock2 in the Fuzheng Huayu group and Fasudil hydrochloride group was lower than that in the model group (P<0.05). Conclusion Fuzheng Huayu Capsule can decrease the expression of Rock1, Rock2 and Rock2 in the marginal zone of myocardial infarction in rats with MI. The anti ventricular remodeling mechanism of Fuzheng Huayu Capsule maybe related with this.关键词
心肌梗死/心肌纤维化/Rock/扶正化瘀胶囊/大鼠Key words
myocardial infarction/myocardial fibrosis/Rock/Fuzheng Huayu Capsule/rats分类
医药卫生引用本文复制引用
张冬梅,赵久丽,吴爱明,娄利霞,赵明镜,吕晞滢,赵一舟,柴立民,高永红,孙逸坤..扶正化瘀胶囊对心肌梗死大鼠Rock1、Rock2表达的影响[J].中国中医药信息杂志,2016,23(9):74-77,4.基金项目
国家自然科学基金（81473662）；北京中医药大学自主创新项目 （）
