中国现代中药2016，Vol.18Issue(7)：818-821,4.DOI:10.13313/j.issn.1673-4890.2016.7.004
半夏曲发酵过程化学成分的 HPLC 特征指纹图谱分析
HPLC Characteristic Fingerprint Analysis of Pinelliae Rhizoma Fermentata in Fermenting Process
摘要
Abstract
Objective:To establish HPLC characteristic fingerprint for different fermented products of Pinelliae Rhizoma Fermentata and provide reference for the research on material foundations.Methods:The HPLC fingerprints of different fermented products of Pinelliae Rhizoma Fermentata were established and analyzed with Similarity Evaluation System for Chromatographic Fingerprint of TCM(Version 2004A).Results:RSD of methodological study in HPLC fingerprint were less than 3.0%,which was correspond to related regulations;Three specific peaks were recognized as guanosine inosine and adenosine.12 chemicals (peak 1,2,3,4,5,6,7,8,9,10,15,19)were always be detected during the fermentation,the more important,there were 7 new ingredients (peak 11,12,13,14,16,17,18)in fermented processing,these seven new peaks increased rapidly,and there were 4 peaks(peak 11,12,13,14),which relative peak areas were very obvious,and reflecting its high ingredients.Conclusion:The change of characteristic fingerprint could reveal that material foundation of Pinelliae Rhizoma Fermentata to a certain extent,which could provide a reference for further study on materials basis.关键词
半夏曲/HPLC/指纹图谱/发酵Key words
Pinelliae Rhizoma Fermentata/HPLC/fingerprint/fermentation引用本文复制引用
张超,卢一,范润勇,兰泽伦,黄勤挽..半夏曲发酵过程化学成分的 HPLC 特征指纹图谱分析[J].中国现代中药,2016,18(7):818-821,4.基金项目
国家中医药管理局2015年公益性行业科研专项项目 （）
