中国现代中药2016，Vol.18Issue(7)：822-825,4.DOI:10.13313/j.issn.1673-4890.2016.7.005
分离筛选淡豆豉发酵菌的培养基优化
Optimization of Selective Medium to Isolate Fermentation Microbes from Sojae Semen Praeparatum
摘要
Abstract
Objective:This study is aimed to optimize the selective medium to isolate Sojae Semen Praeparatum fermentation microbes sciencely,simplely and quickly.Methods:Four conventional medium:potato extract glucose agar medium (PDA),Martin's medium (RA),Czapek 's agar medium (CA),Nutrient agar (NA)were compared with the medicinal medium contained with extract liquid from Mori Folium and Artemisiae Annuae Herba,which are used to culture and isolate fermentation microbes from Sojae Semen Praeparatum at (28 ±2)℃ to observe variation law of microbial flora and proportion.Results:The selective medium contained Mori Folium and Artemisiae Annuae Herba extract liquid was superior to the conventional medium to isolate Sojae Semen Praeparatum fermentatiom microbes.Medicinal medium showed the features of science,simplicity, rapidity and uniqueness.Conclusion: According to the different fermentation medicinal matrix, establishing the method of exclusive and selective medium can also be applied to other fermentation Chinese medicine.关键词
淡豆豉/选择性培养基/发酵菌Key words
Sojae Semen Praeparatum/selective medium/fermentation microbes引用本文复制引用
陈丽艳,刘青,张蕾,方自若,王伟明..分离筛选淡豆豉发酵菌的培养基优化[J].中国现代中药,2016,18(7):822-825,4.基金项目
公益性行业科研专项经费项目 （）
