中国现代中药2016，Vol.18Issue(7)：831-836,845,7.DOI:10.13313/j.issn.1673-4890.2016.7.007
HPLC 法同时测定五倍子发酵百药煎中没食子酸及鞣花酸含量
Determination of Gallic Acid and Ellagic Acid in Gallnut Fermented Chinese Gall Leaven by HPLC
摘要
Abstract
Objective:To determine the gallic acid and ellagic acid in the gallnut fermented Chinese gall leaven by HPLC and compare the content of different batches.Methods:The separation was performed on the Waters Symmmetry ShieldTM RP18 (4.6 ×250 mm,5 μm)C18 column,and acetonitrile-0.1% trifluoroacetic acid aqueous in gradient as mobile phase,the flow rate was 1.0 mL·min -1 ,and the detecting wavelength was set at 254 nm,the column temperature was kept at 35 ℃.Results:The average content of gallic acid and ellagic acid in Chinese gall were 3.94% and 0.25%,respectively, and in Chinese gall leaven were 14.20%,0.26%,respectively.Conclusion:The established method is suitable for the determination of gallic acid and ellagicacid in the gallnut fermented Chinese gall leaven.The results provide a basis for medicinal value research and product development of gallnut fermented Chinese gall leaven.关键词
百药煎/没食子酸/鞣花酸/含量测定Key words
Chinese gall leaven/gallic acid/ellagic acid/determination引用本文复制引用
王瑞生,张振凌,王胜超,李柯柯,孙翼飞..HPLC 法同时测定五倍子发酵百药煎中没食子酸及鞣花酸含量[J].中国现代中药,2016,18(7):831-836,845,7.基金项目
六神曲等7种中药发酵技术及规范化应用研究--百药煎（201507004-03）；呼吸疾病诊疗与新药研发河南省协同创新中心-研究生科研创新基金项目 （）
