中国现代中药2016，Vol.18Issue(7)：837-840,4.DOI:10.13313/j.issn.1673-4890.2016.7.008
胆南星辅料成分分析及其清热作用
Composition Analysis and Compare of Clearing Heat Effects among Arisaema cum Bile Processing
摘要
Abstract
Objective:To compare bile acids composition in pig’s bile,bilis bovina,shee Pbile and differences of clearing heat effect in mice, and provide a reference basis for screening out the arisaema cum bile processing adjuvants.Methods:HPLC-CAD method was used to detect the bile acid composition (cholic acid,deoxidationcholic acid, hyodeoxycholic acid,chenodeoxycholic acid,sodium taurocholic acid)in three kinds of bile.The chromatographic condition was following:Waters C18 column (250 mm ×4.6 mm,5 μm),0.5% Formic acid water (A)-Acetonitrile (B),gradient elution,Volume flow:1 mL·min -1 ,Corona parameters:the nitrogen pressure was 35 psi (241.3 Kpa),the Filter:high, Range 200 pA.The fever model of mice was induce with dry yeast,the effects of clearing the heat on the model among pig’s bile,bilis bovina and shee Pbile were observed and compared.Results:The total amount of the five kinds of bile acids in pig’s bile,bilis bovina,shee Pbile were 1.495 4%,0.354 0% and 1.495 4%.pig’s bile contains the most amount of hyodeoxycholic acid,was 1.128 7%.The 3 kinds of biles all showed clearing heat function.The pig’s bile showed clearing heat effect at 6 h but not persistent.Bilis bovina showed a significant effect of clearing heat at 8 h.Shee Pbile could clear heat significant and persistent.Conclusion:Both the contents of five kinds of bile acids and the effects of clearing heat are different among the pig’s bile,bilis bovina,shee Pbile,In pharmacopoeia we distinguish the Pinellia Cum Bile with three kinds of bile,then we use pharmacodynamic evaluation to filt the which bile fermente Arisaema Cum Bile is best.关键词
胆南星胆汁/高效液相色谱/胆酸/解热Key words
Arisaema cum Bile/HPLC-CAD/cholic acid/clearing heat引用本文复制引用
陈江宁,单国顺,刘晓瑜,高慧..胆南星辅料成分分析及其清热作用[J].中国现代中药,2016,18(7):837-840,4.基金项目
公益性行业科研专项 （）
