中国现代中药2016，Vol.18Issue(7)：841-845,5.DOI:10.13313/j.issn.1673-4890.2016.7.009
当归补血汤作用于四膜虫抗氧化基因--抗氧化药物筛选新方法初探
Effects of Danggui Buxue Decoction on mRNA Expression of GPx4 and TxnRd2 in Tetrahymena Thermophile---A New Method for Anti-oxidative Drug Discovery
摘要
Abstract
Objective:To study the effects of Danggui Buxue decoction on mRNA expression of GPx4 and TxnRd2 in Tetrahymena thermophile.Methods:T.thermophila growth was intervened by using the boil-free granule of traditional Chinese medicine.Four different concentrations of Danggui Buxue decoction were added into culture medium of T.thermophile,and each concentrations with two parallel designs,by setting a group using sterile distilled water as blank control.Processing of gene glutathione peroxidase (GPx4)and thioredoxin reductases (TxnRd2)expression data generated by quantitative real-time RT-PCR.Results:Compared with blank control group,the growth curve of medicine intervention went up significantly.Results also showed that the expression level of GPx4 and TxnRd2 were increased by Danggui Buxue decoction.Conclusion:Danggui Buxue decoction could increase the T.thermophile’s growth curve,which may be related to GPx4 TxnRd2 gene expression.关键词
当归补血汤/抗氧化/嗜热四膜虫/GPx4/TxnRd2Key words
Tetrahymena thermophile/Danggui Buxue decoction/GPx4/TxnRd2引用本文复制引用
李一鸣,王平..当归补血汤作用于四膜虫抗氧化基因--抗氧化药物筛选新方法初探[J].中国现代中药,2016,18(7):841-845,5.基金项目
国家自然科学基金委重点项目 （）
