中国现代中药2016，Vol.18Issue(7)：851-856,865,7.DOI:10.13313/j.issn.1673-4890.2016.7.011
不同生长期酸枣叶中多类型资源性化学成分的积累变化与分析评价
Dynamic Analysis of Nucleosides,Amino Acids and Flavonoids in Leaves of Ziziphus jujuba var.spinosa Collected in Different Growth Period
摘要
Abstract
Objective:To investigate the dynamic changes of multiple ingredients in Ziziphus jujuba var.spinosa leaf (ZSL)collected in different growth period,and provide the data for selection of the suitable harvest time of ZSL.Methods:UPLC-TQ MS and HPLC methods were used to determine the contents of 19 nucleosides and 23 amino acids as well as rutin, hyperoside and isoquercitrin in ZSL and their contents in the samples collected in different growth periods were compared.Results:The content differences of nucleosides,amino acids and flavonoids in ZSL collected in different growing periods were greatly obvious.Among them,the content of nucleosides generally exhibited two peaks with the leaf growth.Amino acids content showed a trend that increased firstly,then decreased.For flavonoids,a gradually reducing trend from the high level of their whole content was found.The total content of these three types constituents were relatively high in samples collected in June 28th.Conclusion:Given the above results,the suitable harvesting time for ZSL should be in late June when it was mainly used to prepare the tea.The results of this study provide a scientific basis for selecting the suitable harvesting period for ZSL as well the data for the efficient utilization of ZSL resource.关键词
酸枣叶/核苷类/氨基酸类/黄酮类/采收期Key words
Ziziphus jujuba var.spinosa leaf/nucleosides/amino acids/flavonoids/harvesting time引用本文复制引用
张颖,郭盛,朱邵晴,严辉,王强,徐璐,段金廒..不同生长期酸枣叶中多类型资源性化学成分的积累变化与分析评价[J].中国现代中药,2016,18(7):851-856,865,7.基金项目
国家自然科学基金面上项目（81473538）；教育部霍英东教育基金会高等院校青年教师基金项目（141040）；国家中医药行业科研专项（201407002）；江苏省中药资源产业化过程协同创新中心重点项目（ZDXM-2-8）；江苏高校优势学科建设工程资助项目 （）
